1gia

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(New page: 200px<br /> <applet load="1gia" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gia, resolution 2.0&Aring;" /> '''STRUCTURE OF ACTIVE ...)
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[[Image:1gia.gif|left|200px]]<br />
 
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<applet load="1gia" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gia, resolution 2.0&Aring;" />
 
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'''STRUCTURE OF ACTIVE CONFORMATIONS OF GIA1 AND THE MECHANISM OF GTP HYDROLYSIS'''<br />
 
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==Overview==
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==STRUCTURE OF ACTIVE CONFORMATIONS OF GIA1 AND THE MECHANISM OF GTP HYDROLYSIS==
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Mechanisms of guanosine triphosphate (GTP) hydrolysis by members of the G, protein alpha subunit-p21ras superfamily of guanosine triphosphatases have, been studied extensively but have not been well understood., High-resolution x-ray structures of the GTP gamma S and GDP.AlF4-, complexes formed by the G protein Gi alpha 1 demonstrate specific roles in, transition-state stabilization for two highly conserved residues., Glutamine204 (Gln61 in p21ras) stabilizes and orients the hydrolytic water, in the trigonal-bipyramidal transition state. Arginine 178 stabilizes the, negative charge at the equatorial oxygen atoms of the pentacoordinate, phosphate intermediate. Conserved only in the G alpha family, this residue, may account for the higher hydrolytic rate of G alpha proteins relative to, those of the p21ras family members. The fold of Gi alpha 1 differs from, that of the homologous Gt alpha subunit in the conformation of a, helix-loop sequence located in the alpha-helical domain that is, characteristic of these proteins; this site may participate in effector, binding. The amino-terminal 33 residues are disordered in GTP gamma S-Gi, alpha 1, suggesting a mechanism that may promote release of the beta gamma, subunit complex when the alpha subunit is activated by GTP.
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<StructureSection load='1gia' size='340' side='right'caption='[[1gia]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1gia]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The October 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''G Proteins'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_10 10.2210/rcsb_pdb/mom_2004_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GIA FirstGlance]. <br>
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1GIA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with MG and GSP as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1GIA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb58_1.html G Proteins]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GIA OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gia OCA], [https://pdbe.org/1gia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gia RCSB], [https://www.ebi.ac.uk/pdbsum/1gia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gia ProSAT]</span></td></tr>
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Structures of active conformations of Gi alpha 1 and the mechanism of GTP hydrolysis., Coleman DE, Berghuis AM, Lee E, Linder ME, Gilman AG, Sprang SR, Science. 1994 Sep 2;265(5177):1405-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8073283 8073283]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GNAI1_RAT GNAI1_RAT] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.<ref>PMID:16870394</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gi/1gia_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gia ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: G Proteins]]
[[Category: G Proteins]]
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Berghuis AM]]
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[[Category: Berghuis, A.M.]]
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[[Category: Coleman DE]]
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[[Category: Coleman, D.E.]]
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[[Category: Sprang SR]]
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[[Category: Sprang, S.R.]]
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[[Category: GSP]]
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[[Category: MG]]
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[[Category: signal transduction protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:00:53 2007''
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STRUCTURE OF ACTIVE CONFORMATIONS OF GIA1 AND THE MECHANISM OF GTP HYDROLYSIS

PDB ID 1gia

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