1jgj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1jgj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jgj, resolution 2.40&Aring;" /> '''CRYSTAL STRUCTURE O...)
Current revision (04:38, 17 October 2024) (edit) (undo)
 
(20 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1jgj.gif|left|200px]]<br />
 
-
<applet load="1jgj" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1jgj, resolution 2.40&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF SENSORY RHODOPSIN II AT 2.4 ANGSTROMS: INSIGHTS INTO COLOR TUNING AND TRANSDUCER INTERACTION'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF SENSORY RHODOPSIN II AT 2.4 ANGSTROMS: INSIGHTS INTO COLOR TUNING AND TRANSDUCER INTERACTION==
-
We report an atomic-resolution structure for a sensory member of the, microbial rhodopsin family, the phototaxis receptor sensory rhodopsin II, (NpSRII), which mediates blue-light avoidance by the haloarchaeon, Natronobacterium pharaonis. The 2.4 angstrom structure reveals features, responsible for the 70- to 80-nanometer blue shift of its absorption, maximum relative to those of haloarchaeal transport rhodopsins, as well as, structural differences due to its sensory, as opposed to transport, function. Multiple factors appear to account for the spectral tuning, difference with respect to bacteriorhodopsin: (i) repositioning of the, guanidinium group of arginine 72, a residue that interacts with the, counterion to the retinylidene protonated Schiff base; (ii) rearrangement, of the protein near the retinal ring; and (iii) changes in tilt and slant, of the retinal polyene chain. Inspection of the surface topography reveals, an exposed polar residue, tyrosine 199, not present in bacteriorhodopsin, in the middle of the membrane bilayer. We propose that this residue, interacts with the adjacent helices of the cognate NpSRII transducer, NpHtrII.
+
<StructureSection load='1jgj' size='340' side='right'caption='[[1jgj]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1jgj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. The March 2002 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Bacteriorhodopsin'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2002_3 10.2210/rcsb_pdb/mom_2002_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JGJ FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jgj OCA], [https://pdbe.org/1jgj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jgj RCSB], [https://www.ebi.ac.uk/pdbsum/1jgj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jgj ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/BACS2_NATPH BACS2_NATPH] Involved in the control of phototaxis. Seems to activate a methyl-accepting protein (HTR-II). Photoreceptor for blue light.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jg/1jgj_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jgj ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
We report an atomic-resolution structure for a sensory member of the microbial rhodopsin family, the phototaxis receptor sensory rhodopsin II (NpSRII), which mediates blue-light avoidance by the haloarchaeon Natronobacterium pharaonis. The 2.4 angstrom structure reveals features responsible for the 70- to 80-nanometer blue shift of its absorption maximum relative to those of haloarchaeal transport rhodopsins, as well as structural differences due to its sensory, as opposed to transport, function. Multiple factors appear to account for the spectral tuning difference with respect to bacteriorhodopsin: (i) repositioning of the guanidinium group of arginine 72, a residue that interacts with the counterion to the retinylidene protonated Schiff base; (ii) rearrangement of the protein near the retinal ring; and (iii) changes in tilt and slant of the retinal polyene chain. Inspection of the surface topography reveals an exposed polar residue, tyrosine 199, not present in bacteriorhodopsin, in the middle of the membrane bilayer. We propose that this residue interacts with the adjacent helices of the cognate NpSRII transducer NpHtrII.
-
==About this Structure==
+
Crystal structure of sensory rhodopsin II at 2.4 angstroms: insights into color tuning and transducer interaction.,Luecke H, Schobert B, Lanyi JK, Spudich EN, Spudich JL Science. 2001 Aug 24;293(5534):1499-503. Epub 2001 Jul 12. PMID:11452084<ref>PMID:11452084</ref>
-
1JGJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis] with BOG and RET as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1JGJ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb27_1.html Bacteriorhodopsin]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JGJ OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structure of sensory rhodopsin II at 2.4 angstroms: insights into color tuning and transducer interaction., Luecke H, Schobert B, Lanyi JK, Spudich EN, Spudich JL, Science. 2001 Aug 24;293(5534):1499-503. Epub 2001 Jul 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11452084 11452084]
+
</div>
 +
<div class="pdbe-citations 1jgj" style="background-color:#fffaf0;"></div>
 +
 
 +
==See Also==
 +
*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
 +
*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Bacteriorhodopsin]]
[[Category: Bacteriorhodopsin]]
 +
[[Category: Large Structures]]
[[Category: Natronomonas pharaonis]]
[[Category: Natronomonas pharaonis]]
-
[[Category: Single protein]]
+
[[Category: RCSB PDB Molecule of the Month]]
-
[[Category: Luecke, H.]]
+
[[Category: Luecke H]]
-
[[Category: BOG]]
+
-
[[Category: RET]]
+
-
[[Category: membrane protein]]
+
-
[[Category: phototaxis receptor]]
+
-
[[Category: sensory rhodopsin]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:02:23 2007''
+

Current revision

CRYSTAL STRUCTURE OF SENSORY RHODOPSIN II AT 2.4 ANGSTROMS: INSIGHTS INTO COLOR TUNING AND TRANSDUCER INTERACTION

PDB ID 1jgj

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jgj"
Personal tools