2oa9

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[[Image:2oa9.jpg|left|200px]]
 
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==Restriction endonuclease MvaI in the absence of DNA==
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The line below this paragraph, containing "STRUCTURE_2oa9", creates the "Structure Box" on the page.
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<StructureSection load='2oa9' size='340' side='right'caption='[[2oa9]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2oa9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kocuria_varians Kocuria varians]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OA9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_2oa9| PDB=2oa9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oa9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oa9 OCA], [https://pdbe.org/2oa9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oa9 RCSB], [https://www.ebi.ac.uk/pdbsum/2oa9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oa9 ProSAT]</span></td></tr>
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</table>
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'''Restriction endonuclease MvaI in the absence of DNA'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q8RNV5_KOCVA Q8RNV5_KOCVA]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/2oa9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oa9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Restriction endonuclease MvaI recognizes the sequence CC/WGG (W stands for A or T, '/' designates the cleavage site) and generates products with single nucleotide 5'-overhangs. The enzyme has been noted for its tolerance towards DNA modifications. Here, we report a biochemical characterization and crystal structures of MvaI in an apo-form and in a complex with target DNA at 1.5 A resolution. Our results show that MvaI is a monomer and recognizes its pseudosymmetric target sequence asymmetrically. The enzyme consists of two lobes. The catalytic lobe anchors the active site residues Glu36, Asp50, Glu55 and Lys57 and contacts the bases from the minor grove side. The recognition lobe mediates all major grove interactions with the bases. The enzyme in the crystal is bound to the strand with T at the center of the recognition sequence. The crystal structure with calcium ions and DNA mimics the prereactive state. MvaI shows structural similarities to BcnI, which cleaves the related sequence CC/SGG and to MutH enzyme, which is a component of the DNA repair machinery, and nicks one DNA strand instead of making a double-strand break.
Restriction endonuclease MvaI recognizes the sequence CC/WGG (W stands for A or T, '/' designates the cleavage site) and generates products with single nucleotide 5'-overhangs. The enzyme has been noted for its tolerance towards DNA modifications. Here, we report a biochemical characterization and crystal structures of MvaI in an apo-form and in a complex with target DNA at 1.5 A resolution. Our results show that MvaI is a monomer and recognizes its pseudosymmetric target sequence asymmetrically. The enzyme consists of two lobes. The catalytic lobe anchors the active site residues Glu36, Asp50, Glu55 and Lys57 and contacts the bases from the minor grove side. The recognition lobe mediates all major grove interactions with the bases. The enzyme in the crystal is bound to the strand with T at the center of the recognition sequence. The crystal structure with calcium ions and DNA mimics the prereactive state. MvaI shows structural similarities to BcnI, which cleaves the related sequence CC/SGG and to MutH enzyme, which is a component of the DNA repair machinery, and nicks one DNA strand instead of making a double-strand break.
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==About this Structure==
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Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically.,Kaus-Drobek M, Czapinska H, Sokolowska M, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M Nucleic Acids Res. 2007;35(6):2035-46. Epub 2007 Mar 7. PMID:17344322<ref>PMID:17344322</ref>
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2OA9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Kocuria_varians Kocuria varians]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OA9 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically., Kaus-Drobek M, Czapinska H, Sokolowska M, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M, Nucleic Acids Res. 2007;35(6):2035-46. Epub 2007 Mar 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17344322 17344322]
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</div>
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<div class="pdbe-citations 2oa9" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Kocuria varians]]
[[Category: Kocuria varians]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bochtler, M.]]
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[[Category: Bochtler M]]
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[[Category: Czapinska, H.]]
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[[Category: Czapinska H]]
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[[Category: Kaus-Drobek, M.]]
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[[Category: Kaus-Drobek M]]
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[[Category: Siksnys, V.]]
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[[Category: Siksnys V]]
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[[Category: Sokolowska, M.]]
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[[Category: Sokolowska M]]
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[[Category: Szczepanowski, R H.]]
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[[Category: Szczepanowski RH]]
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[[Category: Tamulaitis, G.]]
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[[Category: Tamulaitis G]]
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[[Category: Urbanke, K.]]
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[[Category: Urbanke K]]
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[[Category: Monomeric endonuclease]]
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[[Category: Mvai]]
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[[Category: Restriction enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:30:52 2008''
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Current revision

Restriction endonuclease MvaI in the absence of DNA

PDB ID 2oa9

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