1phz

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(New page: 200px<br /> <applet load="1phz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1phz, resolution 2.20&Aring;" /> '''STRUCTURE OF PHOSPH...)
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[[Image:1phz.gif|left|200px]]<br />
 
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<applet load="1phz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1phz, resolution 2.20&Aring;" />
 
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'''STRUCTURE OF PHOSPHORYLATED PHENYLALANINE HYDROXYLASE'''<br />
 
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==Overview==
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==STRUCTURE OF PHOSPHORYLATED PHENYLALANINE HYDROXYLASE==
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Phenylalanine hydroxylase converts phenylalanine to tyrosine, a, rate-limiting step in phenylalanine catabolism and protein and, neurotransmitter biosynthesis. It is tightly regulated by the substrates, phenylalanine and tetrahydrobiopterin and by phosphorylation. We present, the crystal structures of dephosphorylated and phosphorylated forms of a, dimeric enzyme with catalytic and regulatory properties of the wild-type, protein. The structures reveal a catalytic domain flexibly linked to a, regulatory domain. The latter consists of an N-terminal autoregulatory, sequence (containing Ser 16, which is the site of phosphorylation) that, extends over the active site pocket, and an alpha-beta sandwich core that, is, unexpectedly, structurally related to both pterin dehydratase and the, regulatory domains of metabolic enzymes. Phosphorylation has no major, structural effects in the absence of phenylalanine, suggesting that, phenylalanine and phosphorylation act in concert to activate the enzyme, through a combination of intrasteric and possibly allosteric mechanisms.
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<StructureSection load='1phz' size='340' side='right'caption='[[1phz]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1phz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The January 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phenylalanine Hydroxylase'' by Shuchismita Dutta and David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_1 10.2210/rcsb_pdb/mom_2005_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PHZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1phz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1phz OCA], [https://pdbe.org/1phz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1phz RCSB], [https://www.ebi.ac.uk/pdbsum/1phz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1phz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PH4H_RAT PH4H_RAT]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ph/1phz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1phz ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PHZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with FE as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1PHZ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb61_1.html Phenylalanine Hydroxylase]]. Active as [http://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PHZ OCA].
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*[[Hydroxylases 3D structures|Hydroxylases 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis of autoregulation of phenylalanine hydroxylase., Kobe B, Jennings IG, House CM, Michell BJ, Goodwill KE, Santarsiero BD, Stevens RC, Cotton RG, Kemp BE, Nat Struct Biol. 1999 May;6(5):442-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10331871 10331871]
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[[Category: Large Structures]]
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[[Category: Phenylalanine 4-monooxygenase]]
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[[Category: Phenylalanine Hydroxylase]]
[[Category: Phenylalanine Hydroxylase]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Cotton RG]]
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[[Category: Cotton, R.G.]]
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[[Category: House CM]]
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[[Category: House, C.M.]]
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[[Category: Jennings IG]]
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[[Category: Jennings, I.G.]]
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[[Category: Kemp BE]]
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[[Category: Kemp, B.E.]]
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[[Category: Kobe B]]
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[[Category: Kobe, B.]]
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[[Category: Michell BJ]]
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[[Category: Michell, B.J.]]
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[[Category: FE]]
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[[Category: allosteric regulation]]
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[[Category: aromatic amino acid hydroxylase]]
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[[Category: intrasteric regulation]]
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[[Category: phenylalanine hydroxylase]]
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[[Category: phosphorylation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:04:40 2007''
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Current revision

STRUCTURE OF PHOSPHORYLATED PHENYLALANINE HYDROXYLASE

PDB ID 1phz

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