1qys
From Proteopedia
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(New page: 200px<br /> <applet load="1qys" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qys, resolution 2.50Å" /> '''Crystal structure o...) |
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| - | [[Image:1qys.gif|left|200px]]<br /> | ||
| - | <applet load="1qys" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1qys, resolution 2.50Å" /> | ||
| - | '''Crystal structure of Top7: A computationally designed protein with a novel fold'''<br /> | ||
| - | == | + | ==Crystal structure of Top7: A computationally designed protein with a novel fold== |
| - | A major challenge of computational protein design is the creation of novel | + | <StructureSection load='1qys' size='340' side='right'caption='[[1qys]], [[Resolution|resolution]] 2.50Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1qys]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Computationally_Designed_Sequence Computationally Designed Sequence]. The October 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Designer Proteins'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_10 10.2210/rcsb_pdb/mom_2005_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QYS FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qys OCA], [https://pdbe.org/1qys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qys RCSB], [https://www.ebi.ac.uk/pdbsum/1qys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qys ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A major challenge of computational protein design is the creation of novel proteins with arbitrarily chosen three-dimensional structures. Here, we used a general computational strategy that iterates between sequence design and structure prediction to design a 93-residue alpha/beta protein called Top7 with a novel sequence and topology. Top7 was found experimentally to be folded and extremely stable, and the x-ray crystal structure of Top7 is similar (root mean square deviation equals 1.2 angstroms) to the design model. The ability to design a new protein fold makes possible the exploration of the large regions of the protein universe not yet observed in nature. | ||
| - | + | Design of a novel globular protein fold with atomic-level accuracy.,Kuhlman B, Dantas G, Ireton GC, Varani G, Stoddard BL, Baker D Science. 2003 Nov 21;302(5649):1364-8. PMID:14631033<ref>PMID:14631033</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: Computationally | + | <div class="pdbe-citations 1qys" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Computationally Designed Sequence]] | ||
[[Category: Designer Proteins]] | [[Category: Designer Proteins]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Baker | + | [[Category: RCSB PDB Molecule of the Month]] |
| - | [[Category: Dantas | + | [[Category: Baker D]] |
| - | [[Category: Ireton | + | [[Category: Dantas G]] |
| - | [[Category: Kuhlman | + | [[Category: Ireton GC]] |
| - | [[Category: Stoddard | + | [[Category: Kuhlman B]] |
| - | [[Category: Varani | + | [[Category: Stoddard BL]] |
| - | + | [[Category: Varani G]] | |
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Current revision
Crystal structure of Top7: A computationally designed protein with a novel fold
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