1r4i

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1r4i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r4i, resolution 3.10&Aring;" /> '''Crystal Structure o...)
Current revision (04:50, 17 October 2024) (edit) (undo)
 
(19 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1r4i.gif|left|200px]]<br />
 
-
<applet load="1r4i" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1r4i, resolution 3.10&Aring;" />
 
-
'''Crystal Structure of Androgen Receptor DNA-Binding Domain Bound to a Direct Repeat Response Element'''<br />
 
-
==Overview==
+
==Crystal Structure of Androgen Receptor DNA-Binding Domain Bound to a Direct Repeat Response Element==
-
Steroid receptors bind as dimers to a degenerate set of response elements, containing inverted repeats of a hexameric half-site separated by 3 bp of, spacer (IR3). Naturally occurring selective androgen response elements, have recently been identified that resemble direct repeats of the, hexameric half-site (ADR3). The 3D crystal structure of the androgen, receptor (AR) DNA-binding domain bound to a selective ADR3 reveals an, unexpected head-to-head arrangement of the two protomers rather than the, expected head-to-tail arrangement seen in nuclear receptors bound to, response elements of similar geometry. Compared with the glucocorticoid, receptor, the DNA-binding domain dimer interface of the AR has additional, interactions that stabilize the AR dimer and increase the affinity for, nonconsensus response elements. This increased interfacial stability, compared with the other steroid receptors may account for the selective, binding of AR to ADR3 response elements.
+
<StructureSection load='1r4i' size='340' side='right'caption='[[1r4i]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1r4i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The August 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Anabolic Steroids'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_8 10.2210/rcsb_pdb/mom_2007_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R4I FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r4i OCA], [https://pdbe.org/1r4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r4i RCSB], [https://www.ebi.ac.uk/pdbsum/1r4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r4i ProSAT]</span></td></tr>
 +
</table>
 +
== Disease ==
 +
[https://www.uniprot.org/uniprot/ANDR_RAT ANDR_RAT] Note=Defects in Ar are a cause of androgen insensitivity. Rats with this syndrome are called testicular feminized (TFM).<ref>PMID:2341409</ref>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ANDR_RAT ANDR_RAT] Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3 (By similarity).<ref>PMID:17181141</ref> <ref>PMID:17008401</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r4/1r4i_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r4i ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Steroid receptors bind as dimers to a degenerate set of response elements containing inverted repeats of a hexameric half-site separated by 3 bp of spacer (IR3). Naturally occurring selective androgen response elements have recently been identified that resemble direct repeats of the hexameric half-site (ADR3). The 3D crystal structure of the androgen receptor (AR) DNA-binding domain bound to a selective ADR3 reveals an unexpected head-to-head arrangement of the two protomers rather than the expected head-to-tail arrangement seen in nuclear receptors bound to response elements of similar geometry. Compared with the glucocorticoid receptor, the DNA-binding domain dimer interface of the AR has additional interactions that stabilize the AR dimer and increase the affinity for nonconsensus response elements. This increased interfacial stability compared with the other steroid receptors may account for the selective binding of AR to ADR3 response elements.
-
==About this Structure==
+
Structural basis of androgen receptor binding to selective androgen response elements.,Shaffer PL, Jivan A, Dollins DE, Claessens F, Gewirth DT Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):4758-63. Epub 2004 Mar 22. PMID:15037741<ref>PMID:15037741</ref>
-
1R4I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1R4I with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb92_1.html Anabolic Steroids]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R4I OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structural basis of androgen receptor binding to selective androgen response elements., Shaffer PL, Jivan A, Dollins DE, Claessens F, Gewirth DT, Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):4758-63. Epub 2004 Mar 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15037741 15037741]
+
</div>
 +
<div class="pdbe-citations 1r4i" style="background-color:#fffaf0;"></div>
 +
 
 +
==See Also==
 +
*[[Androgen receptor 3D structures|Androgen receptor 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Anabolic Steroids]]
[[Category: Anabolic Steroids]]
 +
[[Category: Large Structures]]
 +
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
-
[[Category: Single protein]]
+
[[Category: Claessens F]]
-
[[Category: Claessens, F.]]
+
[[Category: Dollins DE]]
-
[[Category: Dollins, D.E.]]
+
[[Category: Gewirth DT]]
-
[[Category: Gewirth, D.T.]]
+
[[Category: Jivan A]]
-
[[Category: Jivan, A.]]
+
[[Category: Shaffer PL]]
-
[[Category: Shaffer, P.L.]]
+
-
[[Category: ZN]]
+
-
[[Category: androgen]]
+
-
[[Category: ar]]
+
-
[[Category: protein-dna complex]]
+
-
[[Category: steroid receptor]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:05:19 2007''
+

Current revision

Crystal Structure of Androgen Receptor DNA-Binding Domain Bound to a Direct Repeat Response Element

PDB ID 1r4i

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1r4i"
Personal tools