1ae6

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(New page: 200px<br /> <applet load="1ae6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ae6, resolution 3.00&Aring;" /> '''IGG-FAB FRAGMENT OF...)
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[[Image:1ae6.gif|left|200px]]<br />
 
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<applet load="1ae6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ae6, resolution 3.00&Aring;" />
 
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'''IGG-FAB FRAGMENT OF MOUSE MONOCLONAL ANTIBODY CTM01'''<br />
 
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==Overview==
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==IGG-FAB FRAGMENT OF MOUSE MONOCLONAL ANTIBODY CTM01==
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The crystal structures of two pairs of Fab fragments have been determined., The pairs comprise both a murine and an engineered human form, each, derived from the antitumor antibodies A5B7 and CTM01. Although antigen, specificity is maintained within the pairs, antigen affinity varies. A, comparison of the hypervariable loops for each pair of antibodies shows, their structure has been well maintained in grafting, supporting the, canonical loop model. Detailed structural analysis of the binding sites, and domain arrangements for these antibodies suggests the differences in, antigen affinity observed are likely to be due to inherent flexibility of, the hypervariable loops and movements at the VL:VH domain interface. The, four structures provide the first opportunity to study in detail the, effects of protein engineering on specific antibodies.
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<StructureSection load='1ae6' size='340' side='right'caption='[[1ae6]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ae6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae6 OCA], [https://pdbe.org/1ae6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q99LC4_MOUSE Q99LC4_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structures of two pairs of Fab fragments have been determined. The pairs comprise both a murine and an engineered human form, each derived from the antitumor antibodies A5B7 and CTM01. Although antigen specificity is maintained within the pairs, antigen affinity varies. A comparison of the hypervariable loops for each pair of antibodies shows their structure has been well maintained in grafting, supporting the canonical loop model. Detailed structural analysis of the binding sites and domain arrangements for these antibodies suggests the differences in antigen affinity observed are likely to be due to inherent flexibility of the hypervariable loops and movements at the VL:VH domain interface. The four structures provide the first opportunity to study in detail the effects of protein engineering on specific antibodies.
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==About this Structure==
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VL:VH domain rotations in engineered antibodies: crystal structures of the Fab fragments from two murine antitumor antibodies and their engineered human constructs.,Banfield MJ, King DJ, Mountain A, Brady RL Proteins. 1997 Oct;29(2):161-71. PMID:9329081<ref>PMID:9329081</ref>
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1AE6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AE6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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VL:VH domain rotations in engineered antibodies: crystal structures of the Fab fragments from two murine antitumor antibodies and their engineered human constructs., Banfield MJ, King DJ, Mountain A, Brady RL, Proteins. 1997 Oct;29(2):161-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9329081 9329081]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 1ae6" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Banfield, M.J.]]
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[[Category: Brady, R.L.]]
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[[Category: fab fragment]]
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[[Category: humanisation]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:25:56 2007''
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Banfield MJ]]
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[[Category: Brady RL]]

Current revision

IGG-FAB FRAGMENT OF MOUSE MONOCLONAL ANTIBODY CTM01

PDB ID 1ae6

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