2owy

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[[Image:2owy.jpg|left|200px]]
 
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==The recombination-associated protein RdgC adopts a novel toroidal architecture for DNA binding==
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The line below this paragraph, containing "STRUCTURE_2owy", creates the "Structure Box" on the page.
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<StructureSection load='2owy' size='340' side='right'caption='[[2owy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2owy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OWY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2owy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2owy OCA], [https://pdbe.org/2owy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2owy RCSB], [https://www.ebi.ac.uk/pdbsum/2owy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2owy ProSAT]</span></td></tr>
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{{STRUCTURE_2owy| PDB=2owy | SCENE= }}
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</table>
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== Function ==
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'''The recombination-associated protein RdgC adopts a novel toroidal architecture for DNA binding'''
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[https://www.uniprot.org/uniprot/RDGC_PSEAE RDGC_PSEAE] May be involved in recombination (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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RecA plays a central role in the nonmutagenic repair of stalled replication forks in bacteria. RdgC, a recombination-associated DNA-binding protein, is a potential negative regulator of RecA function. Here, we have determined the crystal structure of RdgC from Pseudomonas aeruginosa. The J-shaped monomer has a unique fold and can be divided into three structural domains: tip domain, center domain and base domain. Two such monomers dimerize to form a ring-shaped molecule of approximate 2-fold symmetry. Of the two inter-subunit interfaces within the dimer, one interface ('interface A') between tip/center domains is more nonpolar than the other ('interface B') between base domains. The structure allows us to propose that the RdgC dimer binds dsDNA through the central hole of approximately 30 A diameter. The proposed model is supported by our DNA-binding assays coupled with mutagenesis, which indicate that the conserved positively charged residues on the protein surface around the central hole play important roles in DNA binding. The novel ring-shaped architecture of the RdgC dimer has significant implications for its role in homologous recombination.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ow/2owy_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2OWY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OWY OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2owy ConSurf].
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The recombination-associated protein RdgC adopts a novel toroidal architecture for DNA binding., Ha JY, Kim HK, Kim do J, Kim KH, Oh SJ, Lee HH, Yoon HJ, Song HK, Suh SW, Nucleic Acids Res. 2007;35(8):2671-81. Epub 2007 Apr 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17426134 17426134]
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<div style="clear:both"></div>
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[[Category: Pseudomonas aeruginosa]]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Suh, S W.]]
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[[Category: Large Structures]]
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[[Category: Homologous recombination]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Suh SW]]
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[[Category: Rdgc]]
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[[Category: Reca]]
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[[Category: Ring-shaped dna binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:50:22 2008''
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Current revision

The recombination-associated protein RdgC adopts a novel toroidal architecture for DNA binding

PDB ID 2owy

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