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2oyy

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[[Image:2oyy.gif|left|200px]]
 
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==HTHP: a hexameric tyrosine-coordinated heme protein==
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The line below this paragraph, containing "STRUCTURE_2oyy", creates the "Structure Box" on the page.
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<StructureSection load='2oyy' size='340' side='right'caption='[[2oyy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2oyy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruegeria_pomeroyi Ruegeria pomeroyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OYY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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{{STRUCTURE_2oyy| PDB=2oyy | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oyy OCA], [https://pdbe.org/2oyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oyy RCSB], [https://www.ebi.ac.uk/pdbsum/2oyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oyy ProSAT]</span></td></tr>
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</table>
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'''HTHP: a hexameric tyrosine-coordinated heme protein'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q5LL55_RUEPO Q5LL55_RUEPO]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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We have cloned, expressed, isolated and characterized a hexameric tyrosine-coordinated heme protein (HTHP) from the marine bacterium Silicibacter pomeroyi. HTHP shows peroxidase and catalase activity and has a high thermal stability. As-isolated HTHP has absorption maxima at 407, 495, 504, 532 and 622 nm wavelength. Upon reduction maxima at 430, 564 and 596 nm wavelength are discernible. The crystal structure of HTHP reveals a hexameric, ring-like arrangement of six monomers. Each monomer binds a solvent accessible heme group, which is stabilized by the interaction of three neighboring monomers. The pocket around the heme distal side is positively charged due to three conserved arginine residues in direct vicinity. The heme iron is penta-coordinated with a tyrosine residue as proximal ligand. The coordinating hydroxyl-group of the tyrosine ligand interacts with the guanidinium group of a nearby arginine residue, an arrangement closely resembling the catalytic dyad found in monofunctional heme-containing catalases and coral allene oxide synthases, which are b-type cytochromes with tyrosine coordination trans to an empty coordination site. Despite the similarity in heme coordination HTHP is functionally and structurally unrelated to catalases and other heme-containing proteins. Its hexameric arrangement, solvent accessible heme binding pocket and heme coordination by tyrosine render HTHP a unique protein with unusual properties. A database search against complete and incomplete genomes shows that the 76 amino acid residues sequence of HTHP is unrelated to characterized proteins, but is homologous to orfs found in a phylogenetically diverse set of bacteria with sequence identities of 30-76%. We therefore propose that HTHP is the prototype of a new class of heme proteins.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oy/2oyy_consurf.spt"</scriptWhenChecked>
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2OYY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Silicibacter_pomeroyi Silicibacter pomeroyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OYY OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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HTHP: a novel class of hexameric, tyrosine-coordinated heme proteins., Jeoung JH, Pippig DA, Martins BM, Wagener N, Dobbek H, J Mol Biol. 2007 May 11;368(4):1122-31. Epub 2007 Mar 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17395199 17395199]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oyy ConSurf].
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[[Category: Silicibacter pomeroyi]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Dobbek, H.]]
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</StructureSection>
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[[Category: All helical]]
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[[Category: Large Structures]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:57:51 2008''
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[[Category: Ruegeria pomeroyi]]
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[[Category: Dobbek H]]

Current revision

HTHP: a hexameric tyrosine-coordinated heme protein

PDB ID 2oyy

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