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1dba

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(New page: 200px<br /> <applet load="1dba" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dba, resolution 2.8&Aring;" /> '''THREE-DIMENSIONAL ST...)
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[[Image:1dba.gif|left|200px]]<br />
 
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<applet load="1dba" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dba, resolution 2.8&Aring;" />
 
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'''THREE-DIMENSIONAL STRUCTURE OF AN ANTI-STEROID FAB' AND PROGESTERONE-FAB' COMPLEX'''<br />
 
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==Overview==
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==THREE-DIMENSIONAL STRUCTURE OF AN ANTI-STEROID FAB' AND PROGESTERONE-FAB' COMPLEX==
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The monoclonal anti-progesterone antibody DB3 binds progesterone with, nanomolar affinity (Ka approximately 10(9) M-1), suggesting high, specificity. However, DB3 also cross-reacts with similar affinity with a, subgroup of structurally distinct, progesterone-like steroids. Crystals of, the unliganded Fab' and various steroid-Fab' complexes are isomorphous and, belong to the hexagonal space group, P6(4)22, with unit cell dimensions of, a = b = 135 A, c = 124 A. Structures of free and progesterone-bound Fab', have been determined by X-ray crystallography at 2.7 A resolution using, molecular replacement techniques. Progesterone is bound in a hydrophobic, pocket formed mainly by the interaction of three complementarity, determining regions L1, H2 and H3. The orientation of the ligand in the, binding site was aided by both crystallographic and biochemical analyses, of substituted steroids. The indole side-chain of TrpH100 of the DB3 has, two different conformations, inter-converting "open" and "closed" forms of, the antibody combining site. The TrpH100 indole thus appears to be acting, as an antibody-derived surrogate ligand for its own hydrophobic binding, pocket. These structures provide the first atomic view of how a steroid, interacts with a protein and offer a structural explanation for the, restriction of the anti-progesterone response to the VGAM3.8 family of VH, genes.
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<StructureSection load='1dba' size='340' side='right'caption='[[1dba]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dba]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DBA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dba OCA], [https://pdbe.org/1dba PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dba RCSB], [https://www.ebi.ac.uk/pdbsum/1dba PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dba ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IGHG1_MOUSE IGHG1_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/1dba_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dba ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1DBA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with EOH as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DBA OCA].
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Sandbox 20009|Sandbox 20009]]
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==Reference==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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Three-dimensional structure of an anti-steroid Fab' and progesterone-Fab' complex., Arevalo JH, Stura EA, Taussig MJ, Wilson IA, J Mol Biol. 1993 May 5;231(1):103-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8496956 8496956]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Arevalo, J.H.]]
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[[Category: Large Structures]]
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[[Category: Wilson, I.A.]]
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[[Category: Mus musculus]]
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[[Category: EOH]]
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[[Category: Arevalo JH]]
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[[Category: immunoglobulin]]
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[[Category: Wilson IA]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:28:10 2007''
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Current revision

THREE-DIMENSIONAL STRUCTURE OF AN ANTI-STEROID FAB' AND PROGESTERONE-FAB' COMPLEX

PDB ID 1dba

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