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2p0m

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[[Image:2p0m.jpg|left|200px]]
 
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==Revised structure of rabbit reticulocyte 15S-lipoxygenase==
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The line below this paragraph, containing "STRUCTURE_2p0m", creates the "Structure Box" on the page.
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<StructureSection load='2p0m' size='340' side='right'caption='[[2p0m]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2p0m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P0M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P0M FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=RS7:(2E)-3-(2-OCT-1-YN-1-YLPHENYL)ACRYLIC+ACID'>RS7</scene></td></tr>
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{{STRUCTURE_2p0m| PDB=2p0m | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p0m OCA], [https://pdbe.org/2p0m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p0m RCSB], [https://www.ebi.ac.uk/pdbsum/2p0m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p0m ProSAT]</span></td></tr>
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</table>
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'''Revised structure of rabbit reticulocyte 15S-lipoxygenase'''
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== Function ==
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[https://www.uniprot.org/uniprot/LOX15_RABIT LOX15_RABIT]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Lipoxygenases (LOXs) are a family of nonheme iron dioxygenases that catalyze the regioselective and stereospecific hydroperoxidation of polyunsaturated fatty acids, and are involved in a variety of inflammatory diseases and cancers. The crystal structure of rabbit 15S-LOX1 that was reported by Gillmor et al. in 1997 has played key roles for understanding the properties of mammalian LOXs. In this structure, three segments, including 12 residues in the superficial alpha2 helix, are absent and have usually been described as "disordered." By reinterpreting the original crystallographic data we were able to elucidate two different conformations of the molecule, both having well ordered alpha2 helices. Surprisingly, one molecule contained an inhibitor and the other did not, thereby adopting a closed and an open form, respectively. They differed in the conformation of the segments that were absent in the original structure, which is highlighted by a 12 A movement of alpha2. Consequently, they showed a difference in the size and shape of the substrate-binding cavity. The new model should provide new insight into the catalytic mechanism involving induced conformational change of the binding pocket. It may also be helpful for the structure-based design of LOX inhibitors.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p0/2p0m_consurf.spt"</scriptWhenChecked>
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2P0M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P0M OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Conformational flexibility in mammalian 15S-lipoxygenase: Reinterpretation of the crystallographic data., Choi J, Chon JK, Kim S, Shin W, Proteins. 2008 Feb 15;70(3):1023-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17847087 17847087]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p0m ConSurf].
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[[Category: Arachidonate 15-lipoxygenase]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
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[[Category: Single protein]]
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[[Category: Choi J]]
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[[Category: Choi, J.]]
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[[Category: Chon JK]]
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[[Category: Chon, J K.]]
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[[Category: Kim S]]
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[[Category: Kim, S.]]
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[[Category: Shin W]]
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[[Category: Shin, W.]]
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[[Category: 15s-lipoxygenase]]
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[[Category: Arachidonate]]
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[[Category: Conformational change]]
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[[Category: Iron]]
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[[Category: Oxidoreductase]]
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[[Category: Pseudo symmetry]]
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[[Category: Rabbit]]
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[[Category: Twin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:04:47 2008''
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Current revision

Revised structure of rabbit reticulocyte 15S-lipoxygenase

PDB ID 2p0m

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