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1emt

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(New page: 200px<br /> <applet load="1emt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1emt, resolution 2.25&Aring;" /> '''FAB ANTIBODY FRAGME...)
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[[Image:1emt.gif|left|200px]]<br />
 
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<applet load="1emt" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1emt, resolution 2.25&Aring;" />
 
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'''FAB ANTIBODY FRAGMENT OF AN C60 ANTIFULLERENE ANTIBODY'''<br />
 
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==Overview==
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==FAB ANTIBODY FRAGMENT OF AN C60 ANTIFULLERENE ANTIBODY==
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We have prepared a monoclonal Buckminsterfullerene specific antibody and, report the sequences of its light and heavy chains. We also show, by x-ray, crystallographic analysis of the Fab fragment and by model building, that, the fullerene binding site is formed by the interface of the antibody, light and heavy chains. Shape-complementary clustering of hydrophobic, amino acids, several of which participate in putative stacking, interactions with fullerene, form the binding site. Moreover, an induced, fit mechanism appears to participate in the fullerene binding process., Affinity of the antibody-fullerene complex is 22 nM as measured by, competitive binding. These findings should be applicable not only to the, use of antibodies to assay and direct potential fullerene-based drug, design but could also lead to new methodologies for the production of, fullerene derivatives and nanotubes as well.
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<StructureSection load='1emt' size='340' side='right'caption='[[1emt]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1emt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EMT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1emt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1emt OCA], [https://pdbe.org/1emt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1emt RCSB], [https://www.ebi.ac.uk/pdbsum/1emt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1emt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/1emt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1emt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EMT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EMT OCA].
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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X-ray crystal structure of an anti-Buckminsterfullerene antibody fab fragment: biomolecular recognition of C(60)., Braden BC, Goldbaum FA, Chen BX, Kirschner AN, Wilson SR, Erlanger BF, Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12193-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11035793 11035793]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Braden BC]]
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[[Category: Braden, B.C.]]
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[[Category: Chen B-X]]
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[[Category: Chen, B.X.]]
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[[Category: Erlanger BF]]
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[[Category: Erlanger, B.F.]]
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[[Category: Goldbaum FA]]
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[[Category: Goldbaum, F.A.]]
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[[Category: Kirschner AN]]
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[[Category: Kirschner, A.N.]]
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[[Category: Wilson SR]]
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[[Category: Wilson, S.R.]]
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[[Category: anti-fullerene antibody]]
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[[Category: nanotubes]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:29:17 2007''
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Current revision

FAB ANTIBODY FRAGMENT OF AN C60 ANTIFULLERENE ANTIBODY

PDB ID 1emt

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