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1eo8

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(New page: 200px<br /> <applet load="1eo8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eo8, resolution 2.8&Aring;" /> '''INFLUENZA VIRUS HEMA...)
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[[Image:1eo8.gif|left|200px]]<br />
 
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<applet load="1eo8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eo8, resolution 2.8&Aring;" />
 
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'''INFLUENZA VIRUS HEMAGGLUTININ COMPLEXED WITH A NEUTRALIZING ANTIBODY'''<br />
 
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==Overview==
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==INFLUENZA VIRUS HEMAGGLUTININ COMPLEXED WITH A NEUTRALIZING ANTIBODY==
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The structure of a complex between the hemagglutinin of influenza virus, and the Fab of a neutralizing antibody was determined by X-ray, crystallography at 2.8 A resolution. This antibody and another which has, only 56% sequence identity bind to the same epitope with very similar, affinities and in the same orientation. One third of the interactions is, conserved in the two complexes; a significant proportion of the, interactions that differ are established by residues of the H3, complementarity-determining regions (CDR) which adopt distinct, conformations in the two antibodies. This demonstrates that there is a, definite flexibility in the selection of antibodies that bind to a given, epitope, despite the high affinity of their complexes. This flexibility, allows the humoral immune response to be redundant, a feature that may be, useful in achieving longer lasting protection against evolving viral, pathogens.
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<StructureSection load='1eo8' size='340' side='right'caption='[[1eo8]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1eo8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/X-31(H3N2)) Influenza A virus (A/X-31(H3N2))] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EO8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EO8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eo8 OCA], [https://pdbe.org/1eo8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eo8 RCSB], [https://www.ebi.ac.uk/pdbsum/1eo8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eo8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HEMA_I68A0 HEMA_I68A0] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/1eo8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eo8 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EO8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EO8 OCA].
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]]
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==Reference==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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Structural evidence for recognition of a single epitope by two distinct antibodies., Fleury D, Daniels RS, Skehel JJ, Knossow M, Bizebard T, Proteins. 2000 Sep 1;40(4):572-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10899782 10899782]
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__TOC__
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[[Category: Influenza a virus]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Bizebard T]]
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[[Category: Bizebard, T.]]
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[[Category: Daniels RS]]
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[[Category: Daniels, R.S.]]
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[[Category: Fleury D]]
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[[Category: Fleury, D.]]
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[[Category: Gigant B]]
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[[Category: Gigant, B.]]
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[[Category: Knossow M]]
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[[Category: Knossow, M.]]
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[[Category: Skehel JJ]]
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[[Category: Skehel, J.J.]]
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[[Category: NAG]]
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[[Category: complex (hemagglutinin/immmunoglobulin)]]
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[[Category: hemagglutinin]]
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[[Category: immune system complex]]
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[[Category: immunoglobulin]]
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[[Category: viral protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:29:21 2007''
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Current revision

INFLUENZA VIRUS HEMAGGLUTININ COMPLEXED WITH A NEUTRALIZING ANTIBODY

PDB ID 1eo8

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