1f11

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(New page: 200px<br /> <applet load="1f11" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f11, resolution 3.0&Aring;" /> '''F124 FAB FRAGMENT FR...)
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[[Image:1f11.gif|left|200px]]<br />
 
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<applet load="1f11" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f11, resolution 3.0&Aring;" />
 
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'''F124 FAB FRAGMENT FROM A MONOCLONAL ANTI-PRES2 ANTIBODY'''<br />
 
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==Overview==
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==F124 FAB FRAGMENT FROM A MONOCLONAL ANTI-PRES2 ANTIBODY==
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The crystal structure of the Fab fragment from the monoclonal anti-preS2, antibody F124 (IgG1,kappa) has been solved by molecular replacement and, refined at 3.0 A resolution. The Fab crystallizes with two independent, molecules in the asymmetric unit. F124 recognizes an epitope contained, within the preS2 segment between residues 120 and 132 of the surface, antigen of hepatitis B virus. The antibody shows a high affinity for the, glycan N-linked to Asn123, but it also cross-reacts with the, non-glycosylated peptide fragment 120-132. Although crystallization was, performed in the presence of an eightfold excess of the cross-reactive, peptide, no evidence for the ligand was found in the antigen-binding site, which is close to a neighbouring molecule in the crystal lattice. The, antigen-binding site has a groove-like topology which is modulated with, pocket-like cavities. It is characterized by a large number of tyrosine, and aspartate residues. The importance of germ-line mutations at the, binding site is discussed.
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<StructureSection load='1f11' size='340' side='right'caption='[[1f11]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f11]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F11 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F11 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f11 OCA], [https://pdbe.org/1f11 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f11 RCSB], [https://www.ebi.ac.uk/pdbsum/1f11 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f11 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KV3AD_MOUSE KV3AD_MOUSE] [https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f1/1f11_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f11 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the Fab fragment from the monoclonal anti-preS2 antibody F124 (IgG1,kappa) has been solved by molecular replacement and refined at 3.0 A resolution. The Fab crystallizes with two independent molecules in the asymmetric unit. F124 recognizes an epitope contained within the preS2 segment between residues 120 and 132 of the surface antigen of hepatitis B virus. The antibody shows a high affinity for the glycan N-linked to Asn123, but it also cross-reacts with the non-glycosylated peptide fragment 120-132. Although crystallization was performed in the presence of an eightfold excess of the cross-reactive peptide, no evidence for the ligand was found in the antigen-binding site, which is close to a neighbouring molecule in the crystal lattice. The antigen-binding site has a groove-like topology which is modulated with pocket-like cavities. It is characterized by a large number of tyrosine and aspartate residues. The importance of germ-line mutations at the binding site is discussed.
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==About this Structure==
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Structure of the Fab fragment from F124, a monoclonal antibody specific for hepatitis B surface antigen.,Saul FA, Vulliez-Le Normand B, Passafiume M, Riottot MM, Bentley GA Acta Crystallogr D Biol Crystallogr. 2000 Aug;56(Pt 8):945-51. PMID:10944330<ref>PMID:10944330</ref>
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1F11 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F11 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of the Fab fragment from F124, a monoclonal antibody specific for hepatitis B surface antigen., Saul FA, Vulliez-Le Normand B, Passafiume M, Riottot MM, Bentley GA, Acta Crystallogr D Biol Crystallogr. 2000 Aug;56(Pt 8):945-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10944330 10944330]
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</div>
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<div class="pdbe-citations 1f11" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Bentley GA]]
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[[Category: Bentley, G.A.]]
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[[Category: Passafiume M]]
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[[Category: Normand, B.Vulliez-Le.]]
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[[Category: Riottot MM]]
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[[Category: Passafiume, M.]]
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[[Category: Saul FA]]
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[[Category: Riottot, M.M.]]
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[[Category: Vulliez-Le Normand B]]
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[[Category: Saul, F.A.]]
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[[Category: antibody]]
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[[Category: fab]]
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[[Category: hepatitis b]]
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[[Category: immunoglobulin]]
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[[Category: pres2]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:29:30 2007''
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Current revision

F124 FAB FRAGMENT FROM A MONOCLONAL ANTI-PRES2 ANTIBODY

PDB ID 1f11

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