2p4b

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[[Image:2p4b.gif|left|200px]]
 
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==Crystal structure of E.coli RseB==
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The line below this paragraph, containing "STRUCTURE_2p4b", creates the "Structure Box" on the page.
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<StructureSection load='2p4b' size='340' side='right'caption='[[2p4b]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2p4b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P4B FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
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{{STRUCTURE_2p4b| PDB=2p4b | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p4b OCA], [https://pdbe.org/2p4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p4b RCSB], [https://www.ebi.ac.uk/pdbsum/2p4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p4b ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of E.coli RseB'''
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== Function ==
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[https://www.uniprot.org/uniprot/RSEB_ECOLI RSEB_ECOLI] Seems to modulate the activity of RpoE (sigma-E).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The bacterial envelope stress response senses stress signals in the extracytoplasmic compartment, and activates sigma(E)-dependent transcription by degrading its antisigma factor RseA. RseB, a binding partner of RseA, plays a pivotal role in regulating this response, but its molecular mechanism is not understood. We therefore determined the crystal structure of Escherichia coli RseB at a resolution of 2.4 A. RseB is composed of two domains linked by a flexible linker and forms a loosely packed dimer with two grooves on each side. This structural feature is confirmed by small-angle scattering in solution. Analysis of the binding of various RseA mutants to RseB allowed us to identify the major RseB-binding motif in RseA. These data, coupled with analysis of small-angle scattering of the RseA/RseB complex in solution, leads us to propose that two RseAs bind to the grooves of the dimeric RseB by conserved residues. The implications for modulating proteolytic cleavage of RseA are discussed.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p4/2p4b_consurf.spt"</scriptWhenChecked>
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2P4B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P4B OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of RseB and a model of its binding mode to RseA., Kim DY, Jin KS, Kwon E, Ree M, Kim KK, Proc Natl Acad Sci U S A. 2007 May 22;104(21):8779-84. Epub 2007 May 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17496148 17496148]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p4b ConSurf].
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[[Category: Escherichia coli]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Kim, D Y.]]
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</StructureSection>
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[[Category: Kim, K K.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Open and closed form]]
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[[Category: Large Structures]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:19:44 2008''
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[[Category: Kim DY]]
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[[Category: Kim KK]]

Current revision

Crystal structure of E.coli RseB

PDB ID 2p4b

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