2p4v

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[[Image:2p4v.jpg|left|200px]]
 
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==Crystal structure of the transcript cleavage factor, GreB at 2.6A resolution==
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The line below this paragraph, containing "STRUCTURE_2p4v", creates the "Structure Box" on the page.
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<StructureSection load='2p4v' size='340' side='right'caption='[[2p4v]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2p4v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P4V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P4V FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p4v OCA], [https://pdbe.org/2p4v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p4v RCSB], [https://www.ebi.ac.uk/pdbsum/2p4v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p4v ProSAT]</span></td></tr>
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{{STRUCTURE_2p4v| PDB=2p4v | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GREB_ECOLI GREB_ECOLI] Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreB releases sequences of up to 9 nucleotides in length.[HAMAP-Rule:MF_00930]<ref>PMID:8431948</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p4/2p4v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p4v ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of the transcript cleavage factor, GreB at 2.6A resolution'''
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==See Also==
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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== References ==
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==Overview==
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<references/>
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Bacterial Gre transcript cleavage factors stimulate the intrinsic endonucleolytic activity of RNA polymerase (RNAP) to rescue stalled transcription complexes. They bind to RNAP and extend their coiled-coil (CC) domains to the catalytic centre through the secondary channel. Three existing models for the Gre-RNAP complex postulate congruent mechanisms of Gre-assisted catalysis, while offering conflicting views of the Gre-RNAP interactions. Here, we report the GreB structure of Escherichia coli. The GreB monomers form a triangle with the tip of the amino-terminal CC of one molecule trapped within the hydrophobic cavity of the carboxy-terminal domain of a second molecule. This arrangement suggests an analogous model for recruitment to RNAP. Indeed, the beta'-subunit CC located at the rim of the secondary channel has conserved hydrophobic residues at its tip. We show that substitutions of these residues and those in the GreB C-terminal domain cavity confer defects in GreB activity and binding to RNAP, and present a plausible model for the RNAP-GreB complex.
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__TOC__
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</StructureSection>
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==About this Structure==
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2P4V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P4V OCA].
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==Reference==
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The carboxy-terminal coiled-coil of the RNA polymerase beta'-subunit is the main binding site for Gre factors., Vassylyeva MN, Svetlov V, Dearborn AD, Klyuyev S, Artsimovitch I, Vassylyev DG, EMBO Rep. 2007 Nov;8(11):1038-43. Epub 2007 Oct 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17917675 17917675]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Artsimovitch, I.]]
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[[Category: Artsimovitch I]]
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[[Category: Dearborn, A D.]]
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[[Category: Dearborn AD]]
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[[Category: Klyuyev, S.]]
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[[Category: Klyuyev S]]
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[[Category: Svetlov, V.]]
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[[Category: Svetlov V]]
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[[Category: Vassylyev, D G.]]
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[[Category: Vassylyev DG]]
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[[Category: Vassylyeva, M N.]]
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[[Category: Vassylyeva MN]]
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[[Category: Crystal structure]]
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[[Category: Gre-factor]]
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[[Category: Rna polymerase]]
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[[Category: Transcript cleavage]]
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[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:21:59 2008''
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Current revision

Crystal structure of the transcript cleavage factor, GreB at 2.6A resolution

PDB ID 2p4v

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