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1fbi

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(New page: 200px<br /> <applet load="1fbi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fbi, resolution 3.0&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1fbi.gif|left|200px]]<br />
 
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<applet load="1fbi" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fbi, resolution 3.0&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A CROSS-REACTION COMPLEX BETWEEN FAB F9.13.7 AND GUINEA-FOWL LYSOZYME'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A CROSS-REACTION COMPLEX BETWEEN FAB F9.13.7 AND GUINEA-FOWL LYSOZYME==
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The crystal structure of the complex between the cross-reacting antigen, Guinea fowl lysozyme and the Fab from monoclonal antibody F9.13.7, raised, against hen egg lysozyme, has been determined by x-ray diffraction to 3-A, resolution. The antibody interacts with exposed residues of an alpha-helix, and surrounding loops adjacent to the lysozyme active site cleft. The, epitope of lysozyme bound by antibody F9.13.7 overlaps almost completely, with that bound by antibody HyHEL10; the same 12 residues of the antigen, interact with the two antibodies. The antibodies, however, have different, combining sites with no sequence homology at any of their, complementarity-determining regions and show a dissimilar pattern of, cross-reactivity with heterologous antigens. Side chain mobility of, epitope residues contributes to confer steric and electrostatic, complementarity to differently shaped combining sites, allowing functional, mimicry to occur. The capacity of two antibodies that have different fine, specificities to bind the same area of the antigen emphasizes the, operational character of the definition of an antigenic determinant. This, example demonstrates that degenerate binding of the same structural motif, does not require the existence of sequence homology or other chemical, similarities between the different binding sites.
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<StructureSection load='1fbi' size='340' side='right'caption='[[1fbi]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fbi]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Numida_meleagris Numida meleagris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FBI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fbi OCA], [https://pdbe.org/1fbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fbi RCSB], [https://www.ebi.ac.uk/pdbsum/1fbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fbi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/1fbi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fbi ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FBI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Numida_meleagris Numida meleagris]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FBI OCA].
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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==Reference==
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__TOC__
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Crystal structure of a cross-reaction complex between Fab F9.13.7 and guinea fowl lysozyme., Lescar J, Pellegrini M, Souchon H, Tello D, Poljak RJ, Peterson N, Greene M, Alzari PM, J Biol Chem. 1995 Jul 28;270(30):18067-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7629116 7629116]
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</StructureSection>
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[[Category: Lysozyme]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Numida meleagris]]
[[Category: Numida meleagris]]
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[[Category: Protein complex]]
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[[Category: Alzari PM]]
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[[Category: Alzari, P.M.]]
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[[Category: Lescar J]]
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[[Category: Lescar, J.]]
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[[Category: complex (antibody/antigen)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:30:02 2007''
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Current revision

CRYSTAL STRUCTURE OF A CROSS-REACTION COMPLEX BETWEEN FAB F9.13.7 AND GUINEA-FOWL LYSOZYME

PDB ID 1fbi

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