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1for

From Proteopedia

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(New page: 200px<br /> <applet load="1for" size="450" color="white" frame="true" align="right" spinBox="true" caption="1for, resolution 2.75&Aring;" /> '''STRUCTURE DETERMINA...)
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[[Image:1for.gif|left|200px]]<br />
 
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<applet load="1for" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1for, resolution 2.75&Aring;" />
 
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'''STRUCTURE DETERMINATION OF AN FAB FRAGMENT THAT NEUTRALIZES HUMAN RHINOVIRUS AND ANALYSIS OF THE FAB-VIRUS COMPLEX'''<br />
 
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==Overview==
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==STRUCTURE DETERMINATION OF AN FAB FRAGMENT THAT NEUTRALIZES HUMAN RHINOVIRUS AND ANALYSIS OF THE FAB-VIRUS COMPLEX==
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The crystal structure of Fab17-IA, an antigen-binding fragment from a, murine immunoglobulin that neutralizes human rhinovirus 14 (HRV14), has, been solved to 2.7 A resolution. Fab17-IA crystallized into three, different space groups depending upon the method used to purify the intact, antibody. The structure was determined by use of molecular and isomorphous, replacement methods. The current model has a crystallographic R-factor of, approximately 19% for 10,192 independent reflections between 8 and 2.7 A., Correlation coefficient calculations showed that the Fab17-IA structure, can be fit into the Fab17-IA/HRV14 image reconstruction density to within, 5 A positional accuracy and to within a few degrees of rotation. The, resulting interface of the docked antibody was examined and showed, extensive charge and shape complementarity with the virus surface that was, supported by site-directed mutagenesis experiments. The success of this, approach validates the utility of combining X-ray crystallography with, cryo-electron microscopy of complex macromolecular assemblies.
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<StructureSection load='1for' size='340' side='right'caption='[[1for]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1for]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FOR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1for FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1for OCA], [https://pdbe.org/1for PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1for RCSB], [https://www.ebi.ac.uk/pdbsum/1for PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1for ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/1for_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1for ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FOR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FOR OCA].
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Sandbox 20009|Sandbox 20009]]
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==Reference==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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Structure determination of an Fab fragment that neutralizes human rhinovirus 14 and analysis of the Fab-virus complex., Liu H, Smith TJ, Lee WM, Mosser AG, Rueckert RR, Olson NH, Cheng RH, Baker TS, J Mol Biol. 1994 Jul 8;240(2):127-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8027997 8027997]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Liu, H.]]
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[[Category: Large Structures]]
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[[Category: Smith, T.J.]]
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[[Category: Mus musculus]]
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[[Category: immunoglobulin]]
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[[Category: Liu H]]
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[[Category: Smith TJ]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:30:31 2007''
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Current revision

STRUCTURE DETERMINATION OF AN FAB FRAGMENT THAT NEUTRALIZES HUMAN RHINOVIRUS AND ANALYSIS OF THE FAB-VIRUS COMPLEX

PDB ID 1for

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