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1hil

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(New page: 200px<br /> <applet load="1hil" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hil, resolution 2.0&Aring;" /> '''STRUCTURAL EVIDENCE ...)
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[[Image:1hil.gif|left|200px]]<br />
 
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<applet load="1hil" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hil, resolution 2.0&Aring;" />
 
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'''STRUCTURAL EVIDENCE FOR INDUCED FIT AS A MECHANISM FOR ANTIGEN-ANTIBODY RECOGNITION'''<br />
 
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==Overview==
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==STRUCTURAL EVIDENCE FOR INDUCED FIT AS A MECHANISM FOR ANTIGEN-ANTIBODY RECOGNITION==
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The three-dimensional structure of a specific antibody (Fab 17/9) to a, peptide immunogen from influenza virus hemagglutinin [HA1(75-110)] and two, independent crystal complexes of this antibody with bound peptide, (TyrP100-LeuP108) have been determined by x-ray crystallographic, techniques at 2.0 A, 2.9 A, and 3.1 A resolution, respectively. The, nonapeptide antigen assumes a type I beta turn in the antibody combining, site and interacts primarily with the Fab hypervariable loops L3, H2, and, H3. Comparison of the bound and unbound Fab structures shows that a major, rearrangement in the H3 loop accompanies antigen binding. This, conformational change results in the creation of a binding pocket for the, beta turn of the peptide, allowing TyrP105 to be accommodated. The, conformation of the peptide bound to the antibody shows similarity to its, cognate sequence in the HA1, suggesting a possible mechanism for the, cross-reactivity of this Fab with monomeric hemagglutinin. The structures, of the free and antigen bound antibodies demonstrate the flexibility of, the antibody combining site and provide an example of induced fit as a, mechanism for antibody-antigen recognition.
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<StructureSection load='1hil' size='340' side='right'caption='[[1hil]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1hil]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HIL FirstGlance]. <br>
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1HIL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HIL OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hil OCA], [https://pdbe.org/1hil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hil RCSB], [https://www.ebi.ac.uk/pdbsum/1hil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hil ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structural evidence for induced fit as a mechanism for antibody-antigen recognition., Rini JM, Schulze-Gahmen U, Wilson IA, Science. 1992 Feb 21;255(5047):959-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1546293 1546293]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/1hil_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hil ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Rini JM]]
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[[Category: Rini, J.M.]]
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[[Category: Wilson IA]]
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[[Category: Wilson, I.A.]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:32:06 2007''
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STRUCTURAL EVIDENCE FOR INDUCED FIT AS A MECHANISM FOR ANTIGEN-ANTIBODY RECOGNITION

PDB ID 1hil

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