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2pbo

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[[Image:2pbo.jpg|left|200px]]
 
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==E27Q mutant of EXO-B-(1,3)-Glucanase from Candida Albicans at 1.85 A==
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The line below this paragraph, containing "STRUCTURE_2pbo", creates the "Structure Box" on the page.
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<StructureSection load='2pbo' size='340' side='right'caption='[[2pbo]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pbo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PBO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pbo OCA], [https://pdbe.org/2pbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pbo RCSB], [https://www.ebi.ac.uk/pdbsum/2pbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pbo ProSAT]</span></td></tr>
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{{STRUCTURE_2pbo| PDB=2pbo | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EXG1_CANAL EXG1_CANAL] Major glucan 1,3-beta-glucosidase required for cell wall integrity. Beta-glucanases participate in the metabolism of beta-glucan, the main structural component of the cell wall. Can also function biosynthetically as a transglycosylase. Functions to deliver glucan from the cell to the extracellular matrix. Does not appear to impact cell wall glucan content of biofilm cells, nor is it necessary for filamentation or biofilm formation. Involved in cell-substrate and cell-cell adhesion. Adhesion to host-cell surfaces is the first critical step during mucosal infection. XOG1 is target of human antimicrobial peptide LL-37 for inhibition of cell adhesion.<ref>PMID:21713010</ref> <ref>PMID:22876186</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pb/2pbo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pbo ConSurf].
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<div style="clear:both"></div>
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'''E27Q mutant of EXO-B-(1,3)-Glucanase from Candida Albicans at 1.85 A'''
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==See Also==
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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== References ==
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==Overview==
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<references/>
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A group of fungal exo-beta-(1,3)-glucanases, including that from the human pathogen Candida albicans (Exg), belong to glycosyl hydrolase family 5 that also includes many bacterial cellulases (endo-beta-1, 4-glucanases). Family members, despite wide sequence variations, share a common mechanism and are characterised by possessing eight invariant residues making up the active site. These include two glutamate residues acting as nucleophile and acid/base, respectively. Exg is an abundant secreted enzyme possessing both hydrolase and transferase activity consistent with a role in cell wall glucan metabolism and possibly morphogenesis. The structures of Exg in both free and inhibited forms have been determined to 1.9 A resolution. A distorted (beta/alpha)8 barrel structure accommodates an active site which is located within a deep pocket, formed when extended loop regions close off a cellulase-like groove. Structural analysis of a covalently bound mechanism-based inhibitor (2-fluoroglucosylpyranoside) and of a transition-state analogue (castanospermine) has identified the binding interactions at the -1 glucose binding site. In particular the carboxylate of Glu27 serves a dominant hydrogen-bonding role. Access by a 1,3-glucan chain to the pocket in Exg can be understood in terms of a change in conformation of the terminal glucose residue from chair to twisted boat. The geometry of the pocket is not, however, well suited for cleavage of 1,4-glycosidic linkages. A second glucose site was identified at the entrance to the pocket, sandwiched between two antiparallel phenylalanine side-chains. This aromatic entrance-way must not only direct substrate into the pocket but also may act as a clamp for an acceptor molecule participating in the transfer reaction.
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__TOC__
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</StructureSection>
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==About this Structure==
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2PBO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PBO OCA].
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==Reference==
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The structure of the exo-beta-(1,3)-glucanase from Candida albicans in native and bound forms: relationship between a pocket and groove in family 5 glycosyl hydrolases., Cutfield SM, Davies GJ, Murshudov G, Anderson BF, Moody PC, Sullivan PA, Cutfield JF, J Mol Biol. 1999 Dec 3;294(3):771-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10610795 10610795]
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[[Category: Candida albicans]]
[[Category: Candida albicans]]
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[[Category: Glucan 1,3-beta-glucosidase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Cutfield JF]]
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[[Category: Cutfield, J F.]]
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[[Category: Cutfield SM]]
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[[Category: Cutfield, S M.]]
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[[Category: Patrick WM]]
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[[Category: Patrick, W M.]]
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[[Category: -1 site]]
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[[Category: Candida albican]]
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[[Category: Carbohydrate binding]]
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[[Category: Exo-glucanase]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:47:37 2008''
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Current revision

E27Q mutant of EXO-B-(1,3)-Glucanase from Candida Albicans at 1.85 A

PDB ID 2pbo

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