2pcy

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[[Image:2pcy.gif|left|200px]]
 
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==THE CRYSTAL STRUCTURE OF POPLAR APOPLASTOCYANIN AT 1.8-ANGSTROMS RESOLUTION. THE GEOMETRY OF THE COPPER-BINDING SITE IS CREATED BY THE POLYPEPTIDE==
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The line below this paragraph, containing "STRUCTURE_2pcy", creates the "Structure Box" on the page.
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<StructureSection load='2pcy' size='340' side='right'caption='[[2pcy]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PCY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pcy OCA], [https://pdbe.org/2pcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pcy RCSB], [https://www.ebi.ac.uk/pdbsum/2pcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pcy ProSAT]</span></td></tr>
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{{STRUCTURE_2pcy| PDB=2pcy | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PLAS1_POPNI PLAS1_POPNI] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/2pcy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pcy ConSurf].
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<div style="clear:both"></div>
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'''THE CRYSTAL STRUCTURE OF POPLAR APOPLASTOCYANIN AT 1.8-ANGSTROMS RESOLUTION. THE GEOMETRY OF THE COPPER-BINDING SITE IS CREATED BY THE POLYPEPTIDE'''
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==See Also==
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*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The three-dimensional structure of apoplastocyanin from poplar leaves (Populus nigra var. italica) has been determined by x-ray diffraction at 1.8-A resolution. The structure closely resembles that of the holoprotein. In particular, the positions of the copper-binding residues in the apo- and holoproteins differ by only 0.1-0.3 A. This indicates that the irregular geometry of the "type 1" copper site is imposed upon the metal atom by the polypeptide moiety. A 180 degrees rotation of one solvent-exposed histidine imidazole ring about C beta-C gamma appears to facilitate access to the copper site. The close structural similarity between apo-, Cu-(II)-, and Cu(I)-plastocyanin was initially demonstrated by means of electron density difference maps. Two series of restrained least squares refinement calculations for apoplastocyanin, originating from different sets of atomic positional parameters, were carried out in parallel. Both refinements converged to the same model which, when fully refined, had a residual R = 0.16. Forty-two water molecules were located during the refinement.
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[[Category: Large Structures]]
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==About this Structure==
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2PCY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCY OCA].
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==Reference==
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The crystal structure of poplar apoplastocyanin at 1.8-A resolution. The geometry of the copper-binding site is created by the polypeptide., Garrett TP, Clingeleffer DJ, Guss JM, Rogers SJ, Freeman HC, J Biol Chem. 1984 Mar 10;259(5):2822-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/6698995 6698995]
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[[Category: Populus nigra]]
[[Category: Populus nigra]]
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[[Category: Single protein]]
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[[Category: Freeman HC]]
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[[Category: Freeman, H C.]]
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[[Category: Garrett TPJ]]
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[[Category: Garrett, T P.J.]]
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[[Category: Guss JM]]
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[[Category: Guss, J M.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:52:45 2008''
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Current revision

THE CRYSTAL STRUCTURE OF POPLAR APOPLASTOCYANIN AT 1.8-ANGSTROMS RESOLUTION. THE GEOMETRY OF THE COPPER-BINDING SITE IS CREATED BY THE POLYPEPTIDE

PDB ID 2pcy

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