1k4d

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(New page: 200px<br /> <applet load="1k4d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k4d, resolution 2.30&Aring;" /> '''Potassium Channel K...)
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[[Image:1k4d.gif|left|200px]]<br />
 
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<applet load="1k4d" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k4d, resolution 2.30&Aring;" />
 
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'''Potassium Channel KcsA-Fab complex in low concentration of K+'''<br />
 
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==Overview==
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==Potassium Channel KcsA-Fab complex in low concentration of K+==
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Ion transport proteins must remove an ion's hydration shell to coordinate, the ion selectively on the basis of its size and charge. To discover how, the K+ channel solves this fundamental aspect of ion conduction, we solved, the structure of the KcsA K+ channel in complex with a monoclonal Fab, antibody fragment at 2.0 A resolution. Here we show how the K+ channel, displaces water molecules around an ion at its extracellular entryway, and, how it holds a K+ ion in a square antiprism of water molecules in a cavity, near its intracellular entryway. Carbonyl oxygen atoms within the, selectivity filter form a very similar square antiprism around each K+, binding site, as if to mimic the waters of hydration. The selectivity, filter changes its ion coordination structure in low K+ solutions. This, structural change is crucial to the operation of the selectivity filter in, the cellular context, where the K+ ion concentration near the selectivity, filter varies in response to channel gating.
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<StructureSection load='1k4d' size='340' side='right'caption='[[1k4d]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k4d]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K4D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k4d OCA], [https://pdbe.org/1k4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k4d RCSB], [https://www.ebi.ac.uk/pdbsum/1k4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k4d ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IGHG1_MOUSE IGHG1_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/1k4d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k4d ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1K4D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans] with K, NA, DGA and F09 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K4D OCA].
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Potassium Channel|Potassium Channel]]
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==Reference==
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution., Zhou Y, Morais-Cabral JH, Kaufman A, MacKinnon R, Nature. 2001 Nov 1;414(6859):43-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11689936 11689936]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
 
[[Category: Streptomyces lividans]]
[[Category: Streptomyces lividans]]
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[[Category: Kaufman, A.]]
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[[Category: Kaufman A]]
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[[Category: MacKinnon, R.]]
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[[Category: MacKinnon R]]
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[[Category: Morais-Cabral, J.H.]]
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[[Category: Morais-Cabral JH]]
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[[Category: Zhou, Y.]]
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[[Category: Zhou Y]]
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[[Category: DGA]]
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[[Category: F09]]
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[[Category: K]]
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[[Category: NA]]
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[[Category: k channel]]
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[[Category: protein-antibody fab complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:34:24 2007''
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Current revision

Potassium Channel KcsA-Fab complex in low concentration of K+

PDB ID 1k4d

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