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1kir

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(New page: 200px<br /> <applet load="1kir" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kir, resolution 2.0&Aring;" /> '''FV MUTANT Y(A 50)S (...)
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[[Image:1kir.gif|left|200px]]<br />
 
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<applet load="1kir" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kir, resolution 2.0&Aring;" />
 
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'''FV MUTANT Y(A 50)S (VL DOMAIN) OF MOUSE MONOCLONAL ANTIBODY D1.3 COMPLEXED WITH HEN EGG WHITE LYSOZYME'''<br />
 
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==Overview==
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==FV MUTANT Y(A 50)S (VL DOMAIN) OF MOUSE MONOCLONAL ANTIBODY D1.3 COMPLEXED WITH HEN EGG WHITE LYSOZYME==
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Using site-directed mutagenesis, X-ray crystallography, and titration, calorimetry, we have examined the structural and thermodynamic, consequences of removing specific hydrogen bonds in an antigen-antibody, interface. Crystal structures of three antibody FvD1.3 mutants, VLTyr50Ser, (VLY50S), VHTyr32Ala (VHY32A), and VHTyr101Phe (VHY101F), bound to hen egg, white lysozyme (HEL) have been determined at resolutions ranging from 1.85, to 2.10 A. In the wild-type (WT) FvD1.3-HEL complex, the hydroxyl groups, of VLTyr50, VHTyr32, and VHTyr101 each form at least one hydrogen bond, with the lysozyme antigen. Thermodynamic parameters for antibody-antigen, association have been measured using isothermal titration calorimetry, giving equilibrium binding constants Kb (M-1) of 2.6 x 10(7) (VLY50S), 7.0, x 10(7) (VHY32A), and 4.0 x 10(6) (VHY101F). For the WT complex, Kb is 2.7, x 10(8) M-1; thus, the affinities of the mutant Fv fragments for HEL are, 10-, 4-, and 70-fold lower than that of the original antibody, respectively. In all three cases entropy compensation results in an, affinity loss that would otherwise be larger. Comparison of the three, mutant crystal structures with the WT structure demonstrates that the, removal of direct antigen-antibody hydrogen bonds results in minimal, shifts in the positions of the remaining protein atoms. These observations, show that this complex is considerably tolerant, both structurally and, thermodynamically, to the truncation of antibody side chains that form, hydrogen bonds with the antigen. Alterations in interface solvent, structure for two of the mutant complexes (VLY50S and VHY32A) appear to, compensate for the unfavorable enthalpy changes when protein-protein, interactions are removed. These changes in solvent structure, along with, the increased mobility of side chains near the mutation site, probably, contribute to the observed entropy compensation. For the VHY101F complex, the nature of the large entropy compensation is not evident from a, structural comparison of the WT and mutant complexes. Differences in the, local structure and dynamics of the uncomplexed Fv molecules may account, for the entropic discrepancy in this case.
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<StructureSection load='1kir' size='340' side='right'caption='[[1kir]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kir]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KIR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kir OCA], [https://pdbe.org/1kir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kir RCSB], [https://www.ebi.ac.uk/pdbsum/1kir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kir ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HVM44_MOUSE HVM44_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ki/1kir_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kir ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KIR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KIR OCA].
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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==Reference==
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__TOC__
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Hydrogen bonding and solvent structure in an antigen-antibody interface. Crystal structures and thermodynamic characterization of three Fv mutants complexed with lysozyme., Fields BA, Goldbaum FA, Dall'Acqua W, Malchiodi EL, Cauerhff A, Schwarz FP, Ysern X, Poljak RJ, Mariuzza RA, Biochemistry. 1996 Dec 3;35(48):15494-503. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8952503 8952503]
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</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Lysozyme]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Fields BA]]
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[[Category: Fields, B.A.]]
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[[Category: Mariuzza RA]]
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[[Category: Mariuzza, R.A.]]
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[[Category: Poljak RJ]]
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[[Category: Poljak, R.J.]]
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[[Category: bacteriolytic enzyme]]
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[[Category: complex (immunoglobulin/hydrolase)]]
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[[Category: egg white]]
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[[Category: glycosidase]]
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[[Category: hydrolase]]
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[[Category: immunoglobulin v region]]
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[[Category: signal]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:35:06 2007''
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Current revision

FV MUTANT Y(A 50)S (VL DOMAIN) OF MOUSE MONOCLONAL ANTIBODY D1.3 COMPLEXED WITH HEN EGG WHITE LYSOZYME

PDB ID 1kir

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