2pkg

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:10, 21 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2pkg.gif|left|200px]]
 
-
<!--
+
==Structure of a complex between the A subunit of protein phosphatase 2A and the small t antigen of SV40==
-
The line below this paragraph, containing "STRUCTURE_2pkg", creates the "Structure Box" on the page.
+
<StructureSection load='2pkg' size='340' side='right'caption='[[2pkg]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2pkg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Macaca_mulatta_polyomavirus_1 Macaca mulatta polyomavirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PKG FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_2pkg| PDB=2pkg | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pkg OCA], [https://pdbe.org/2pkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pkg RCSB], [https://www.ebi.ac.uk/pdbsum/2pkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pkg ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/2AAA_HUMAN 2AAA_HUMAN] The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.<ref>PMID:16580887</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pk/2pkg_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pkg ConSurf].
 +
<div style="clear:both"></div>
-
'''Structure of a complex between the A subunit of protein phosphatase 2A and the small t antigen of SV40'''
+
==See Also==
-
 
+
*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]]
-
 
+
== References ==
-
==Overview==
+
<references/>
-
The small t antigen (ST) of DNA tumor virus SV40 facilitates cellular transformation by disrupting the functions of protein phosphatase 2A (PP2A) through a poorly defined mechanism. The crystal structure of the core domain of SV40 ST bound to the scaffolding subunit of human PP2A reveals that the ST core domain has a novel zinc-binding fold and interacts with the conserved ridge of HEAT repeats 3-6, which overlaps with the binding site for the B' (also called PR61 or B56) regulatory subunit. ST has a lower binding affinity than B' for the PP2A core enzyme. Consequently, ST does not efficiently displace B' from PP2A holoenzymes in vitro. Notably, ST inhibits PP2A phosphatase activity through its N-terminal J domain. These findings suggest that ST may function mainly by inhibiting the phosphatase activity of the PP2A core enzyme, and to a lesser extent by modulating assembly of the PP2A holoenzymes.
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
2PKG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PKG OCA].
+
-
 
+
-
==Reference==
+
-
Structural and biochemical insights into the regulation of protein phosphatase 2A by small t antigen of SV40., Chen Y, Xu Y, Bao Q, Xing Y, Li Z, Lin Z, Stock JB, Jeffrey PD, Shi Y, Nat Struct Mol Biol. 2007 Jun;14(6):527-34. Epub 2007 May 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17529992 17529992]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Simian virus 40]]
+
[[Category: Macaca mulatta polyomavirus 1]]
-
[[Category: Jeffrey, P D.]]
+
[[Category: Jeffrey PD]]
-
[[Category: Shi, Y.]]
+
[[Category: Shi Y]]
-
[[Category: Protein phosphatase 2a]]
+
-
[[Category: Regulation]]
+
-
[[Category: Small t antigen]]
+
-
[[Category: Sv40]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:17:50 2008''
+

Current revision

Structure of a complex between the A subunit of protein phosphatase 2A and the small t antigen of SV40

PDB ID 2pkg

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2pkg"
Personal tools