1mel

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(New page: 200px<br /> <applet load="1mel" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mel, resolution 2.5&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1mel.gif|left|200px]]<br />
 
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<applet load="1mel" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mel, resolution 2.5&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A CAMEL SINGLE-DOMAIN VH ANTIBODY FRAGMENT IN COMPLEX WITH LYSOZYME'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A CAMEL SINGLE-DOMAIN VH ANTIBODY FRAGMENT IN COMPLEX WITH LYSOZYME==
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The Camelidae is the only taxonomic family known to possess functional, heavy-chain antibodies, lacking light chains. We report here the 2.5 A, resolution crystal structure of a camel VH in complex with its antigen, lysozyme. Compared to human and mouse VH domains, there are no major, backbone rearrangements in the VH framework. However, the architecture of, the region of VH that interacts with a VL in a conventional FV is, different from any previously seen. Moreover, the CDR1 region, although in, sequence homologous to human CDR1, deviates fundamentally from the, canonical structure. Additionally, one half of the CDR3 contacts the VH, region which in conventional immunoglobulins interacts with a VL whereas, the other half protrudes from the antigen binding site and penetrates, deeply into the active site of lysozyme.
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<StructureSection load='1mel' size='340' side='right'caption='[[1mel]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mel]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. The April 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Nanobodies'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_4 10.2210/rcsb_pdb/mom_2011_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MEL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mel OCA], [https://pdbe.org/1mel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mel RCSB], [https://www.ebi.ac.uk/pdbsum/1mel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mel ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/me/1mel_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mel ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Camelidae is the only taxonomic family known to possess functional heavy-chain antibodies, lacking light chains. We report here the 2.5 A resolution crystal structure of a camel VH in complex with its antigen, lysozyme. Compared to human and mouse VH domains, there are no major backbone rearrangements in the VH framework. However, the architecture of the region of VH that interacts with a VL in a conventional FV is different from any previously seen. Moreover, the CDR1 region, although in sequence homologous to human CDR1, deviates fundamentally from the canonical structure. Additionally, one half of the CDR3 contacts the VH region which in conventional immunoglobulins interacts with a VL whereas the other half protrudes from the antigen binding site and penetrates deeply into the active site of lysozyme.
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==About this Structure==
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Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme.,Desmyter A, Transue TR, Ghahroudi MA, Thi MH, Poortmans F, Hamers R, Muyldermans S, Wyns L Nat Struct Biol. 1996 Sep;3(9):803-11. PMID:8784355<ref>PMID:8784355</ref>
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1MEL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MEL OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme., Desmyter A, Transue TR, Ghahroudi MA, Thi MH, Poortmans F, Hamers R, Muyldermans S, Wyns L, Nat Struct Biol. 1996 Sep;3(9):803-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8784355 8784355]
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</div>
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<div class="pdbe-citations 1mel" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Camelus dromedarius]]
[[Category: Camelus dromedarius]]
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Lysozyme]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Nanobodies]]
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[[Category: Desmyter, A.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Gharoudi, M.Arbabi.]]
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[[Category: Arbabi Gharoudi M]]
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[[Category: Hamers, R.]]
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[[Category: Dao Thi M]]
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[[Category: Muyldermans, S.]]
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[[Category: Desmyter A]]
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[[Category: Poortmans, F.]]
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[[Category: Hamers R]]
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[[Category: Thi, M.Dao.]]
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[[Category: Muyldermans S]]
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[[Category: Transue, T.R.]]
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[[Category: Poortmans F]]
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[[Category: Wyns, L.]]
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[[Category: Transue TR]]
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[[Category: camel single-domain anti-lysozyme]]
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[[Category: Wyns L]]
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[[Category: complex (antibody/antigen)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:36:19 2007''
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Current revision

CRYSTAL STRUCTURE OF A CAMEL SINGLE-DOMAIN VH ANTIBODY FRAGMENT IN COMPLEX WITH LYSOZYME

PDB ID 1mel

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