1mqk

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(New page: 200px<br /> <applet load="1mqk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mqk, resolution 1.28&Aring;" /> '''Crystal structure o...)
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[[Image:1mqk.gif|left|200px]]<br />
 
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<applet load="1mqk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mqk, resolution 1.28&Aring;" />
 
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'''Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2'''<br />
 
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==Overview==
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==Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2==
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The antibody Fv fragment 7E2 has previously been employed in the induced, crystallization of the integral membrane protein cytochrome c oxidase from, Paracoccus denitrificans. The 1.3 A X-ray structure of the uncomplexed, antibody fragment reveals conserved water networks on the surfaces of the, framework regions. A novel consensus motif for water coordination, XX(S/T), is found along the edges of the beta-sandwich, where a water, molecule forms hydrogen bonds to the carbonyl O atom of a residue at, position N and the OG hydroxyl groups of conserved serines or threonines, at position N + 2. Multiple conformations were found in the hydrophobic, core for residues IleL21, LeuL33 and the disulfide bridges. An internal, water molecule that is compatible with only one of the three packing, states of the V(L) core suggests local 'breathing' of the variable domain., TrpH47, a conserved key residue of the V(H)/V(L) interface, is crucially, involved in the formation of the antigen-binding site by adopting a novel, conformation that specifically stabilizes the non-canonical CDR-L3 loop., Finally, a comparison with 7E2-cytochrome c oxidase complexes demonstrates, that binding of this membrane-bound antigen proceeds without major, conformational changes of the 7E2 antibody fragment.
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<StructureSection load='1mqk' size='340' side='right'caption='[[1mqk]], [[Resolution|resolution]] 1.28&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mqk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MQK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.28&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mqk OCA], [https://pdbe.org/1mqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mqk RCSB], [https://www.ebi.ac.uk/pdbsum/1mqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mqk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KV5A4_MOUSE KV5A4_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mq/1mqk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mqk ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MQK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MQK OCA].
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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1.3 A X-ray structure of an antibody Fv fragment used for induced membrane-protein crystallization., Essen LO, Harrenga A, Ostermeier C, Michel H, Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):677-87. Epub 2003, Mar 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12657787 12657787]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Essen L-O]]
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[[Category: Essen, L.O.]]
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[[Category: Harrenga A]]
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[[Category: Harrenga, A.]]
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[[Category: Michel H]]
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[[Category: Michel, H.]]
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[[Category: Ostermeier C]]
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[[Category: Ostermeier, C.]]
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[[Category: antibody]]
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[[Category: cytochrome c oxidase]]
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[[Category: high-resolution structure]]
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[[Category: membrane protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:37:12 2007''
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Current revision

Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2

PDB ID 1mqk

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