2pox

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:29, 30 October 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2pox.jpg|left|200px]]
 
-
<!--
+
==Dark state structure of the reversibly switchable fluorescent protein Dronpa==
-
The line below this paragraph, containing "STRUCTURE_2pox", creates the "Structure Box" on the page.
+
<StructureSection load='2pox' size='340' side='right'caption='[[2pox]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2pox]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._SC22 Echinophyllia sp. SC22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2POX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2POX FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.946&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
-
{{STRUCTURE_2pox| PDB=2pox | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pox OCA], [https://pdbe.org/2pox PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pox RCSB], [https://www.ebi.ac.uk/pdbsum/2pox PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pox ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/po/2pox_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pox ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state by irradiation with light. To elucidate the molecular basis of the switching mechanism, we generated reversibly switchable Dronpa protein crystals. Using these crystals we determined the elusive dark-state structure of Dronpa at 1.95-A resolution. We found that the photoswitching results in a cis-trans isomerization of the chromophore accompanied by complex structural rearrangements of four nearby amino acid residues. Because of this cascade of intramolecular events, the chromophore is exposed to distinct electrostatic surface potentials, which are likely to influence the protonation equilibria at the chromophore. We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore.
-
'''Dark state structure of the reversibly switchable fluorescent protein Dronpa'''
+
Structural basis for reversible photoswitching in Dronpa.,Andresen M, Stiel AC, Trowitzsch S, Weber G, Eggeling C, Wahl MC, Hell SW, Jakobs S Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13005-9. Epub 2007 Jul 23. PMID:17646653<ref>PMID:17646653</ref>
-
 
+
-
 
+
-
==Overview==
+
-
Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state by irradiation with light. To elucidate the molecular basis of the switching mechanism, we generated reversibly switchable Dronpa protein crystals. Using these crystals we determined the elusive dark-state structure of Dronpa at 1.95-A resolution. We found that the photoswitching results in a cis-trans isomerization of the chromophore accompanied by complex structural rearrangements of four nearby amino acid residues. Because of this cascade of intramolecular events, the chromophore is exposed to distinct electrostatic surface potentials, which are likely to influence the protonation equilibria at the chromophore. We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore.
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
2POX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2POX OCA].
+
</div>
 +
<div class="pdbe-citations 2pox" style="background-color:#fffaf0;"></div>
-
==Reference==
+
==See Also==
-
Structural basis for reversible photoswitching in Dronpa., Andresen M, Stiel AC, Trowitzsch S, Weber G, Eggeling C, Wahl MC, Hell SW, Jakobs S, Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13005-9. Epub 2007 Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17646653 17646653]
+
*[[Dronpa|Dronpa]]
-
[[Category: Echinophyllia sp. sc22]]
+
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
-
[[Category: Single protein]]
+
== References ==
-
[[Category: Trowitzsch, S.]]
+
<references/>
-
[[Category: Wahl, M C.]]
+
__TOC__
-
[[Category: Weber, G.]]
+
</StructureSection>
-
[[Category: Green-fluorescent protein-like protein]]
+
[[Category: Echinophyllia sp. SC22]]
-
[[Category: Luminescent protein]]
+
[[Category: Large Structures]]
-
[[Category: Reversibly switchable fluorescent protein]]
+
[[Category: Trowitzsch S]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:33:44 2008''
+
[[Category: Wahl MC]]
 +
[[Category: Weber G]]

Current revision

Dark state structure of the reversibly switchable fluorescent protein Dronpa

PDB ID 2pox

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2pox"
Personal tools