1ngx

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(New page: 200px<br /> <applet load="1ngx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ngx, resolution 1.80&Aring;" /> '''Chimeric Germline F...)
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[[Image:1ngx.gif|left|200px]]<br />
 
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<applet load="1ngx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ngx, resolution 1.80&Aring;" />
 
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'''Chimeric Germline Fab 7g12 with jeffamine fragment bound'''<br />
 
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==Overview==
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==Chimeric Germline Fab 7g12 with jeffamine fragment bound==
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The crystal structure of the Michaelis complex between the Fab fragment of, ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been, solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly, adopts a nonplanar conformation and reveals that the antibody catalyzes, the porphyrin metallation reaction by straining/distorting the bound, substrate toward the transition-state configuration. The crystal, structures of the Fab fragment of the germ-line precursor antibody to 7G12, and its complex with the hapten N-methylmesoporphyrin have also been, solved. A comparison of these structures with the corresponding structures, of the affinity-matured antibody 7G12 reveals the molecular mechanism by, which the immune system evolves binding energy to catalyze this reaction.
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<StructureSection load='1ngx' size='340' side='right'caption='[[1ngx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ngx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NGX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JEF:O-(O-(2-AMINOPROPYL)-O-(2-METHOXYETHYL)POLYPROPYLENE+GLYCOL+500)'>JEF</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ngx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngx OCA], [https://pdbe.org/1ngx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ngx RCSB], [https://www.ebi.ac.uk/pdbsum/1ngx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngx ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ng/1ngx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ngx ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NGX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with JEF as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NGX OCA].
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12552112 12552112]
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[[Category: Homo sapiens]]
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[[Category: ]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Mus musculus]]
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[[Category: Andryski, S.E.]]
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[[Category: Andryski SE]]
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[[Category: Beuscher, A.B.]]
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[[Category: Beuscher AB]]
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[[Category: Schultz, P.G.]]
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[[Category: Schultz PG]]
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[[Category: Stevens, R.C.]]
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[[Category: Stevens RC]]
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[[Category: Yin, J.]]
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[[Category: Yin J]]
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[[Category: JEF]]
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[[Category: antibody]]
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[[Category: antigen binding fragment (fab)]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:38:29 2007''
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Current revision

Chimeric Germline Fab 7g12 with jeffamine fragment bound

PDB ID 1ngx

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