2pqi

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[[Image:2pqi.jpg|left|200px]]
 
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==Crystal structure of active ribosome inactivating protein from maize (b-32)==
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The line below this paragraph, containing "STRUCTURE_2pqi", creates the "Structure Box" on the page.
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<StructureSection load='2pqi' size='340' side='right'caption='[[2pqi]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pqi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PQI FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pqi OCA], [https://pdbe.org/2pqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pqi RCSB], [https://www.ebi.ac.uk/pdbsum/2pqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pqi ProSAT]</span></td></tr>
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{{STRUCTURE_2pqi| PDB=2pqi | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIPX_MAIZE RIPX_MAIZE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pq/2pqi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pqi ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Maize ribosome-inactivating protein is classified as a class III or an atypical RNA N-glycosidase. It is synthesized as an inactive precursor with a 25-amino acid internal inactivation region, which is removed in the active form. As the first structural example of this class of proteins, crystals of the precursor and the active form were diffracted to 2.4 and 2.5 A, respectively. The two proteins are similar, with main chain root mean square deviation (RMSD) of 0.519. In the precursor, the inactivation region is found on the protein surface and consists of a flexible loop followed by a long alpha-helix. This region diminished both the interaction with ribosome and cytotoxicity, but not cellular uptake. Like bacterial ribosome-inactivating proteins, maize ribosome-inactivating protein does not have a back-up glutamate in the active site, which helps the protein to retain some activity if the catalytic glutamate is mutated. The structure reveals that the active site is too small to accommodate two glutamate residues. Our structure suggests that maize ribosome-inactivating protein may represent an intermediate product in the evolution of ribosome-inactivating proteins.
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'''Crystal structure of active ribosome inactivating protein from maize (b-32)'''
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Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site.,Mak AN, Wong YT, An YJ, Cha SS, Sze KH, Au SW, Wong KB, Shaw PC Nucleic Acids Res. 2007;35(18):6259-67. Epub 2007 Sep 13. PMID:17855394<ref>PMID:17855394</ref>
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==Overview==
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Maize ribosome-inactivating protein is classified as a class III or an atypical RNA N-glycosidase. It is synthesized as an inactive precursor with a 25-amino acid internal inactivation region, which is removed in the active form. As the first structural example of this class of proteins, crystals of the precursor and the active form were diffracted to 2.4 and 2.5 A, respectively. The two proteins are similar, with main chain root mean square deviation (RMSD) of 0.519. In the precursor, the inactivation region is found on the protein surface and consists of a flexible loop followed by a long alpha-helix. This region diminished both the interaction with ribosome and cytotoxicity, but not cellular uptake. Like bacterial ribosome-inactivating proteins, maize ribosome-inactivating protein does not have a back-up glutamate in the active site, which helps the protein to retain some activity if the catalytic glutamate is mutated. The structure reveals that the active site is too small to accommodate two glutamate residues. Our structure suggests that maize ribosome-inactivating protein may represent an intermediate product in the evolution of ribosome-inactivating proteins.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2PQI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PQI OCA].
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</div>
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<div class="pdbe-citations 2pqi" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site., Mak AN, Wong YT, An YJ, Cha SS, Sze KH, Au SW, Wong KB, Shaw PC, Nucleic Acids Res. 2007;35(18):6259-67. Epub 2007 Sep 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17855394 17855394]
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*[[Ribosome inactivating protein 3D structures|Ribosome inactivating protein 3D structures]]
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[[Category: Single protein]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Zea mays]]
[[Category: Zea mays]]
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[[Category: RRNA N-glycosylase]]
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[[Category: Au SWN]]
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[[Category: Au, S W.N.]]
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[[Category: Cha SS]]
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[[Category: Cha, S S.]]
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[[Category: Mak ANS]]
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[[Category: Mak, A N.S.]]
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[[Category: Shaw PC]]
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[[Category: Shaw, P C.]]
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[[Category: Sze KH]]
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[[Category: Sze, K H.]]
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[[Category: Wong KB]]
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[[Category: Wong, K B.]]
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[[Category: Wong YT]]
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[[Category: Wong, Y T.]]
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[[Category: Young JA]]
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[[Category: Young, J A.]]
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[[Category: Hydrolase]]
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[[Category: Maize]]
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[[Category: Mod]]
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[[Category: Plant defense]]
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[[Category: Protein synthesis inhibitor]]
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[[Category: Ribosome inactivating protein]]
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[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:37:53 2008''
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Current revision

Crystal structure of active ribosome inactivating protein from maize (b-32)

PDB ID 2pqi

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