1sbs

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(New page: 200px<br /> <applet load="1sbs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sbs, resolution 2.0&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1sbs.gif|left|200px]]<br />
 
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<applet load="1sbs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sbs, resolution 2.0&Aring;" />
 
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'''CRYSTAL STRUCTURE OF AN ANTI-HCG FAB'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF AN ANTI-HCG FAB==
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3A2 is an antibody raised against human chorionic gonadotropin and, recognizes a linear epitope on the C-terminal peptide of the human, chorionic gonadotropin beta-subunit. Its three-dimensional structure has, been determined to 2-A resolution using molecular replacement and refined, to a conventional R-factor of 18.2%. The protein exhibits the typical, immunoglobulin fold, and the model contains 944 ordered water molecules, and one sulfate ion. A comparison of the complementarity-determining, regions of the Fab3A2 with those from the Protein Data Bank following the, canonical structure method reveals a canonical main chain conformation., This antibody belongs to the canonical structure class (combination of, canonical conformations of the complementarity determining loops) that, shows a preference for haptens and not for peptides. However, the shape of, the surface of the antigen binding loops resembles that of an anti-peptide, antibody.
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<StructureSection load='1sbs' size='340' side='right'caption='[[1sbs]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sbs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SBS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sbs OCA], [https://pdbe.org/1sbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sbs RCSB], [https://www.ebi.ac.uk/pdbsum/1sbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sbs ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sb/1sbs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sbs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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3A2 is an antibody raised against human chorionic gonadotropin and recognizes a linear epitope on the C-terminal peptide of the human chorionic gonadotropin beta-subunit. Its three-dimensional structure has been determined to 2-A resolution using molecular replacement and refined to a conventional R-factor of 18.2%. The protein exhibits the typical immunoglobulin fold, and the model contains 944 ordered water molecules and one sulfate ion. A comparison of the complementarity-determining regions of the Fab3A2 with those from the Protein Data Bank following the canonical structure method reveals a canonical main chain conformation. This antibody belongs to the canonical structure class (combination of canonical conformations of the complementarity determining loops) that shows a preference for haptens and not for peptides. However, the shape of the surface of the antigen binding loops resembles that of an anti-peptide antibody.
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==About this Structure==
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Structure of an Fab fragment against a C-terminal peptide of hCG at 2.0 A resolution.,Fotinou C, Beauchamp J, Emsley P, deHaan A, Schielen WJ, Bos E, Isaacs NW J Biol Chem. 1998 Aug 28;273(35):22515-8. PMID:9712877<ref>PMID:9712877</ref>
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1SBS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SBS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of an Fab fragment against a C-terminal peptide of hCG at 2.0 A resolution., Fotinou C, Beauchamp J, Emsley P, deHaan A, Schielen WJ, Bos E, Isaacs NW, J Biol Chem. 1998 Aug 28;273(35):22515-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9712877 9712877]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 1sbs" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Beauchamp, J.]]
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[[Category: Bos, E.]]
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[[Category: Dehaan, A.]]
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[[Category: Emsley, P.]]
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[[Category: Fotinou, C.]]
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[[Category: Isaacs, N.W.]]
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[[Category: Schielen, W.J.G.]]
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[[Category: SO4]]
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[[Category: fab-fragment]]
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[[Category: monoclonal antibody]]
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[[Category: reproduction]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:42:16 2007''
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Beauchamp J]]
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[[Category: Bos E]]
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[[Category: Dehaan A]]
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[[Category: Emsley P]]
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[[Category: Fotinou C]]
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[[Category: Isaacs NW]]
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[[Category: Schielen WJG]]

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CRYSTAL STRUCTURE OF AN ANTI-HCG FAB

PDB ID 1sbs

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