1t6v
From Proteopedia
(Difference between revisions)
(New page: 200px<br /> <applet load="1t6v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t6v, resolution 1.70Å" /> '''Crystal structure a...) |
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| - | [[Image:1t6v.gif|left|200px]]<br /> | ||
| - | <applet load="1t6v" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1t6v, resolution 1.70Å" /> | ||
| - | '''Crystal structure analysis of the nurse shark new antigen receptor (NAR) variable domain in complex with lysozyme'''<br /> | ||
| - | == | + | ==Crystal structure analysis of the nurse shark new antigen receptor (NAR) variable domain in complex with lysozyme== |
| - | Cartilaginous fish are the phylogenetically oldest living organisms known | + | <StructureSection load='1t6v' size='340' side='right'caption='[[1t6v]], [[Resolution|resolution]] 1.70Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1t6v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Ginglymostoma_cirratum Ginglymostoma cirratum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T6V FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t6v OCA], [https://pdbe.org/1t6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t6v RCSB], [https://www.ebi.ac.uk/pdbsum/1t6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t6v ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t6/1t6v_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t6v ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies. | ||
| - | + | Crystal structure of a shark single-domain antibody V region in complex with lysozyme.,Stanfield RL, Dooley H, Flajnik MF, Wilson IA Science. 2004 Sep 17;305(5691):1770-3. Epub 2004 Aug 19. PMID:15319492<ref>PMID:15319492</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 1t6v" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Lysozyme 3D structures|Lysozyme 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Ginglymostoma cirratum]] | [[Category: Ginglymostoma cirratum]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | + | [[Category: Dooley H]] | |
| - | [[Category: Dooley | + | [[Category: Flajnik MF]] |
| - | [[Category: Flajnik | + | [[Category: Stanfield RL]] |
| - | [[Category: Stanfield | + | [[Category: Wilson IA]] |
| - | [[Category: Wilson | + | |
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Current revision
Crystal structure analysis of the nurse shark new antigen receptor (NAR) variable domain in complex with lysozyme
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