2q6o

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[[Image:2q6o.jpg|left|200px]]
 
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==SalL-Y70T with SAM and Cl==
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The line below this paragraph, containing "STRUCTURE_2q6o", creates the "Structure Box" on the page.
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<StructureSection load='2q6o' size='340' side='right'caption='[[2q6o]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2q6o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salinispora_tropica_CNB-440 Salinispora tropica CNB-440]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q6O FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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{{STRUCTURE_2q6o| PDB=2q6o | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6o OCA], [https://pdbe.org/2q6o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q6o RCSB], [https://www.ebi.ac.uk/pdbsum/2q6o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6o ProSAT]</span></td></tr>
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</table>
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'''SalL-Y70T with SAM and Cl'''
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== Function ==
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[https://www.uniprot.org/uniprot/SALL_SALTO SALL_SALTO] Involved in the biosynthesis of the proteosome inhibitor salinosporamide A (SalA). Catalyzes the halogenation of S-adenosyl-L-methionine (SAM) with chloride to generate 5'-chloro-5'-deoxyadenosine (5'-CIDA) and L-methionine. It can also use bromide and iodide, producing halogenated 5'-deoxyadenosine (5'-XDA) and L-methionine, however no halogenase activity is detected in the presence of fluoride.<ref>PMID:18059261</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Halogen atom incorporation into a scaffold of bioactive compounds often amplifies biological activity, as is the case for the anticancer agent salinosporamide A (1), a chlorinated natural product from the marine bacterium Salinispora tropica. Significant effort in understanding enzymatic chlorination shows that oxidative routes predominate to form reactive electrophilic or radical chlorine species. Here we report the genetic, biochemical and structural characterization of the chlorinase SalL, which halogenates S-adenosyl-L-methionine (2) with chloride to generate 5'-chloro-5'-deoxyadenosine (3) and L-methionine (4) in a rarely observed nucleophilic substitution strategy analogous to that of Streptomyces cattleya fluorinase. Further metabolic tailoring produces a halogenated polyketide synthase substrate specific for salinosporamide A biosynthesis. SalL also accepts bromide and iodide as substrates, but not fluoride. High-resolution crystal structures of SalL and active site mutants complexed with substrates and products support the S(N)2 nucleophilic substitution mechanism and further illuminate halide specificity in this newly discovered halogenase family.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q6/2q6o_consurf.spt"</scriptWhenChecked>
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2Q6O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6O OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Discovery and characterization of a marine bacterial SAM-dependent chlorinase., Eustaquio AS, Pojer F, Noel JP, Moore BS, Nat Chem Biol. 2008 Jan;4(1):69-74. Epub 2007 Dec 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18059261 18059261]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q6o ConSurf].
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[[Category: Bacteria]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Noel, J P.]]
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<references/>
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[[Category: Pojer, F.]]
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__TOC__
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[[Category: Biosynthetic protein]]
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</StructureSection>
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[[Category: Chlorinase]]
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[[Category: Large Structures]]
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[[Category: Y70t mutant sam and cl complex]]
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[[Category: Salinispora tropica CNB-440]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:27:00 2008''
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[[Category: Noel JP]]
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[[Category: Pojer F]]

Current revision

SalL-Y70T with SAM and Cl

PDB ID 2q6o

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