2qac

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[[Image:2qac.gif|left|200px]]
 
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==The closed MTIP-MyosinA-tail complex from the malaria parasite invasion machinery==
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The line below this paragraph, containing "STRUCTURE_2qac", creates the "Structure Box" on the page.
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<StructureSection load='2qac' size='340' side='right'caption='[[2qac]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2qac]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7] and [https://en.wikipedia.org/wiki/Plasmodium_yoelii_yoelii Plasmodium yoelii yoelii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QAC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAC:N-ACETYL-SERINE'>SAC</scene></td></tr>
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{{STRUCTURE_2qac| PDB=2qac | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qac OCA], [https://pdbe.org/2qac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qac RCSB], [https://www.ebi.ac.uk/pdbsum/2qac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qac ProSAT]</span></td></tr>
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</table>
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'''The closed MTIP-MyosinA-tail complex from the malaria parasite invasion machinery'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q8I4W8_PLAF7 Q8I4W8_PLAF7]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qa/2qac_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qac ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The Myosin A-tail interacting protein (MTIP) of the malaria parasite links the actomyosin motor of the host cell invasion machinery to its inner membrane complex. We report here that at neutral pH Plasmodium falciparum MTIP in complex with Myosin A adopts a compact conformation, with its two domains completely surrounding the Myosin A-tail helix, dramatically different from previously observed extended MTIP structures. Crystallographic and mutagenesis studies show that H810 and K813 of Myosin A are key players in the formation of the compact MTIP:Myosin A complex. Only the unprotonated state of Myosin A-H810 is compatible with the compact complex. Most surprisingly, every side-chain atom of Myosin A-K813 is engaged in contacts with MTIP. While this side-chain was previously considered to prevent a compact conformation of MTIP with Myosin A, it actually appears to be essential for the formation of the compact complex. The hydrophobic pockets and adaptability seen in the available series of MTIP structures bodes well for the discovery of inhibitors of cell invasion by malaria parasites.
The Myosin A-tail interacting protein (MTIP) of the malaria parasite links the actomyosin motor of the host cell invasion machinery to its inner membrane complex. We report here that at neutral pH Plasmodium falciparum MTIP in complex with Myosin A adopts a compact conformation, with its two domains completely surrounding the Myosin A-tail helix, dramatically different from previously observed extended MTIP structures. Crystallographic and mutagenesis studies show that H810 and K813 of Myosin A are key players in the formation of the compact MTIP:Myosin A complex. Only the unprotonated state of Myosin A-H810 is compatible with the compact complex. Most surprisingly, every side-chain atom of Myosin A-K813 is engaged in contacts with MTIP. While this side-chain was previously considered to prevent a compact conformation of MTIP with Myosin A, it actually appears to be essential for the formation of the compact complex. The hydrophobic pockets and adaptability seen in the available series of MTIP structures bodes well for the discovery of inhibitors of cell invasion by malaria parasites.
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==About this Structure==
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The closed MTIP-myosin A-tail complex from the malaria parasite invasion machinery.,Bosch J, Turley S, Roach CM, Daly TM, Bergman LW, Hol WG J Mol Biol. 2007 Sep 7;372(1):77-88. Epub 2007 Jun 9. PMID:17628590<ref>PMID:17628590</ref>
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2QAC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Plasmodium_falciparum_3d7 Plasmodium falciparum 3d7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QAC OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The closed MTIP-myosin A-tail complex from the malaria parasite invasion machinery., Bosch J, Turley S, Roach CM, Daly TM, Bergman LW, Hol WG, J Mol Biol. 2007 Sep 7;372(1):77-88. Epub 2007 Jun 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17628590 17628590]
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</div>
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[[Category: Plasmodium falciparum 3d7]]
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<div class="pdbe-citations 2qac" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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== References ==
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[[Category: Bergman, L W.]]
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<references/>
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[[Category: Bosch, J.]]
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__TOC__
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[[Category: Daly, T M.]]
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</StructureSection>
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[[Category: Hol, W G.J.]]
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[[Category: Large Structures]]
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[[Category: Roach, C M.]]
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[[Category: Plasmodium falciparum 3D7]]
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[[Category: SGPP, Structural Genomics of Pathogenic Protozoa Consortium.]]
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[[Category: Plasmodium yoelii yoelii]]
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[[Category: Turley, S.]]
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[[Category: Bergman LW]]
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[[Category: Malaria invasion]]
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[[Category: Bosch J]]
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[[Category: Protein structure initiative]]
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[[Category: Daly TM]]
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[[Category: Psi]]
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[[Category: Hol WGJ]]
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[[Category: Sgpp]]
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[[Category: Roach CM]]
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[[Category: Structural genomic]]
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[[Category: Turley S]]
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[[Category: Structural genomics of pathogenic protozoa consortium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:37:43 2008''
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Current revision

The closed MTIP-MyosinA-tail complex from the malaria parasite invasion machinery

PDB ID 2qac

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