2qby

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[[Image:2qby.gif|left|200px]]
 
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==Crystal structure of a heterodimer of Cdc6/Orc1 initiators bound to origin DNA (from S. solfataricus)==
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The line below this paragraph, containing "STRUCTURE_2qby", creates the "Structure Box" on the page.
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<StructureSection load='2qby' size='340' side='right'caption='[[2qby]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2qby]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QBY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene></td></tr>
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{{STRUCTURE_2qby| PDB=2qby | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qby OCA], [https://pdbe.org/2qby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qby RCSB], [https://www.ebi.ac.uk/pdbsum/2qby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qby ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CDC61_SACS2 CDC61_SACS2] Involved in regulation of DNA replication. May play essential roles in origin recognition and cell cycle control of replication. Binds to DNA, with a preference for molecules that contain a bubble, a fork, or a tail. Inhibits the binding of the MCM helicase to the origin DNA and strongly inhibits its DNA helicase activity. Also regulates the DNA polymerase and the nuclease activities of PolB1. Stimulates the DNA-binding activity of Cdc6-3.[HAMAP-Rule:MF_01407]<ref>PMID:16179962</ref> <ref>PMID:17673179</ref> <ref>PMID:17825793</ref> <ref>PMID:19416914</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qb/2qby_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qby ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The faithful duplication of genetic material depends on essential DNA replication initiation factors. Cellular initiators form higher-order assemblies on replication origins, using adenosine triphosphate (ATP) to locally remodel duplex DNA and facilitate proper loading of synthetic replisomal components. To better understand initiator function, we determined the 3.4 angstrom-resolution structure of an archaeal Cdc6/Orc1 heterodimer bound to origin DNA. The structure demonstrates that, in addition to conventional DNA binding elements, initiators use their AAA+ ATPase domains to recognize origin DNA. Together these interactions establish the polarity of initiator assembly on the origin and induce substantial distortions into origin DNA strands. Biochemical and comparative analyses indicate that AAA+/DNA contacts observed in the structure are dynamic and evolutionarily conserved, suggesting that the complex forms a core component of the basal initiation machinery.
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'''Crystal structure of a heterodimer of Cdc6/Orc1 initiators bound to origin DNA (from S. solfataricus)'''
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Replication origin recognition and deformation by a heterodimeric archaeal Orc1 complex.,Dueber EL, Corn JE, Bell SD, Berger JM Science. 2007 Aug 31;317(5842):1210-3. PMID:17761879<ref>PMID:17761879</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2QBY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QBY OCA].
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<div class="pdbe-citations 2qby" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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== References ==
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[[Category: Sulfolobus solfataricus]]
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<references/>
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[[Category: Bell, S D.]]
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__TOC__
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[[Category: Berger, J M.]]
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</StructureSection>
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[[Category: Corn, J E.]]
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[[Category: Large Structures]]
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[[Category: Dueber, E L.Cunningham.]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Aaa+ atpase domain]]
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[[Category: Bell SD]]
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[[Category: Double helix]]
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[[Category: Berger JM]]
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[[Category: Helix-turn-helix]]
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[[Category: Corn JE]]
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[[Category: Protein-dna complex]]
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[[Category: Cunningham Dueber EL]]
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[[Category: Replication/dna complex]]
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[[Category: Winged-helix domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:42:02 2008''
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Current revision

Crystal structure of a heterodimer of Cdc6/Orc1 initiators bound to origin DNA (from S. solfataricus)

PDB ID 2qby

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