2qju

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[[Image:2qju.jpg|left|200px]]
 
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==Crystal Structure of an NSS Homolog with Bound Antidepressant==
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The line below this paragraph, containing "STRUCTURE_2qju", creates the "Structure Box" on the page.
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<StructureSection load='2qju' size='340' side='right'caption='[[2qju]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2qju]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QJU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QJU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSM:3-(10,11-DIHYDRO-5H-DIBENZO[B,F]AZEPIN-5-YL)-N-METHYLPROPAN-1-AMINE'>DSM</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_2qju| PDB=2qju | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qju OCA], [https://pdbe.org/2qju PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qju RCSB], [https://www.ebi.ac.uk/pdbsum/2qju PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qju ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O67854_AQUAE O67854_AQUAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qj/2qju_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qju ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Tricyclic antidepressants exert their pharmacological effect-inhibiting the reuptake of serotonin, norepinephrine, and dopamine-by directly blocking neurotransmitter transporters (SERT, NET, and DAT, respectively) in the presynaptic membrane. The drug-binding site and the mechanism of this inhibition are poorly understood. We determined the crystal structure at 2.9 angstroms of the bacterial leucine transporter (LeuT), a homolog of SERT, NET, and DAT, in complex with leucine and the antidepressant desipramine. Desipramine binds at the inner end of the extracellular cavity of the transporter and is held in place by a hairpin loop and by a salt bridge. This binding site is separated from the leucine-binding site by the extracellular gate of the transporter. By directly locking the gate, desipramine prevents conformational changes and blocks substrate transport. Mutagenesis experiments on human SERT and DAT indicate that both the desipramine-binding site and its inhibition mechanism are probably conserved in the human neurotransmitter transporters.
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'''Crystal Structure of an NSS Homolog with Bound Antidepressant'''
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LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake.,Zhou Z, Zhen J, Karpowich NK, Goetz RM, Law CJ, Reith ME, Wang DN Science. 2007 Sep 7;317(5843):1390-3. Epub 2007 Aug 9. PMID:17690258<ref>PMID:17690258</ref>
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==Overview==
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Tricyclic antidepressants exert their pharmacological effect-inhibiting the reuptake of serotonin, norepinephrine, and dopamine-by directly blocking neurotransmitter transporters (SERT, NET, and DAT, respectively) in the presynaptic membrane. The drug-binding site and the mechanism of this inhibition are poorly understood. We determined the crystal structure at 2.9 angstroms of the bacterial leucine transporter (LeuT), a homolog of SERT, NET, and DAT, in complex with leucine and the antidepressant desipramine. Desipramine binds at the inner end of the extracellular cavity of the transporter and is held in place by a hairpin loop and by a salt bridge. This binding site is separated from the leucine-binding site by the extracellular gate of the transporter. By directly locking the gate, desipramine prevents conformational changes and blocks substrate transport. Mutagenesis experiments on human SERT and DAT indicate that both the desipramine-binding site and its inhibition mechanism are probably conserved in the human neurotransmitter transporters.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2QJU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QJU OCA].
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</div>
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<div class="pdbe-citations 2qju" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake., Zhou Z, Zhen J, Karpowich NK, Goetz RM, Law CJ, Reith ME, Wang DN, Science. 2007 Sep 7;317(5843):1390-3. Epub 2007 Aug 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17690258 17690258]
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*[[Leucine transporter|Leucine transporter]]
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[[Category: Aquifex aeolicus]]
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*[[Symporter 3D structures|Symporter 3D structures]]
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[[Category: Single protein]]
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== References ==
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[[Category: Karpowich, N K.]]
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<references/>
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[[Category: Wang, D N.]]
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__TOC__
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[[Category: Zhou, Z.]]
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</StructureSection>
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[[Category: Antidepressant]]
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[[Category: Aquifex aeolicus VF5]]
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[[Category: Integral membrane protein]]
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[[Category: Large Structures]]
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[[Category: Neurotransmitter]]
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[[Category: Karpowich NK]]
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[[Category: Nss]]
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[[Category: Wang DN]]
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[[Category: Transmembrane transport]]
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[[Category: Zhou Z]]
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[[Category: Transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:04:48 2008''
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Current revision

Crystal Structure of an NSS Homolog with Bound Antidepressant

PDB ID 2qju

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