2qm7

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[[Image:2qm7.jpg|left|200px]]
 
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==MeaB, A Bacterial Homolog of MMAA, Bound to GDP==
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The line below this paragraph, containing "STRUCTURE_2qm7", creates the "Structure Box" on the page.
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<StructureSection load='2qm7' size='340' side='right'caption='[[2qm7]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2qm7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens_AM1 Methylorubrum extorquens AM1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QM7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QM7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_2qm7| PDB=2qm7 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qm7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qm7 OCA], [https://pdbe.org/2qm7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qm7 RCSB], [https://www.ebi.ac.uk/pdbsum/2qm7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qm7 ProSAT]</span></td></tr>
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</table>
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'''MeaB, A Bacterial Homolog of MMAA, Bound to GDP'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q8RPA0_METEX Q8RPA0_METEX]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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MeaB is an auxiliary protein that plays a crucial role in the protection and assembly of the B(12)-dependent enzyme methylmalonyl-CoA mutase. Impairments in the human homologue of MeaB, MMAA, lead to methylmalonic aciduria, an inborn error of metabolism. To explore the role of this metallochaperone, its structure was solved in the nucleotide-free form, as well as in the presence of product, GDP. MeaB is a homodimer, with each subunit containing a central alpha/beta-core G domain that is typical of the GTPase family, as well as alpha-helical extensions at the N and C termini that are not found in other metalloenzyme chaperone GTPases. The C-terminal extension appears to be essential for nucleotide-independent dimerization, and the N-terminal region is implicated in protein-protein interaction with its partner protein, methylmalonyl-CoA mutase. The structure of MeaB confirms that it is a member of the G3E family of P-loop GTPases, which contains other putative metallochaperones HypB, CooC, and UreG. Interestingly, the so-called switch regions, responsible for signal transduction following GTP hydrolysis, are found at the dimer interface of MeaB instead of being positioned at the surface of the protein where its partner protein methylmalonyl-CoA mutase should bind. This observation suggests a large conformation change of MeaB must occur between the GDP- and GTP-bound forms of this protein. Because of their high sequence homology, the missense mutations in MMAA that result in methylmalonic aciduria have been mapped onto MeaB and, in conjunction with mutagenesis data, provide possible explanations for the pathology of this disease.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qm/2qm7_consurf.spt"</scriptWhenChecked>
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2QM7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QM7 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure and mutagenesis of the metallochaperone MeaB: insight into the causes of methylmalonic aciduria., Hubbard PA, Padovani D, Labunska T, Mahlstedt SA, Banerjee R, Drennan CL, J Biol Chem. 2007 Oct 26;282(43):31308-16. Epub 2007 Aug 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17728257 17728257]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qm7 ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Thermomicrobium roseum]]
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__TOC__
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[[Category: Banerjee, R.]]
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</StructureSection>
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[[Category: Drennan, C L.]]
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[[Category: Large Structures]]
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[[Category: Hubbard, P A.]]
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[[Category: Methylorubrum extorquens AM1]]
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[[Category: Labunska, T.]]
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[[Category: Banerjee R]]
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[[Category: Mahlstedt, S A.]]
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[[Category: Drennan CL]]
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[[Category: Padovani, D.]]
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[[Category: Hubbard PA]]
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[[Category: G protein]]
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[[Category: Labunska T]]
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[[Category: G3e]]
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[[Category: Mahlstedt SA]]
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[[Category: Gtpase]]
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[[Category: Padovani D]]
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[[Category: Metallochaperone]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:11:34 2008''
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Current revision

MeaB, A Bacterial Homolog of MMAA, Bound to GDP

PDB ID 2qm7

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