2gsg

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(New page: 200px<br /> <applet load="2gsg" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gsg, resolution 2.10&Aring;" /> '''Crystal structure o...)
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[[Image:2gsg.gif|left|200px]]<br />
 
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<applet load="2gsg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gsg, resolution 2.10&Aring;" />
 
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'''Crystal structure of the Fv fragment of a monoclonal antibody specific for poly-glutamine'''<br />
 
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==Overview==
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==Crystal structure of the Fv fragment of a monoclonal antibody specific for poly-glutamine==
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Huntington and related neurological diseases result from expansion of a, polyglutamine (polyQ) tract. The linear lattice model for the structure, and binding properties of polyQ proposes that both expanded and normal, polyQ tracts in the preaggregation state are random-coil structures but, that an expanded polyQ repeat contains a larger number of epitopes, recognized by antibodies or other proteins. The crystal structure of polyQ, bound to MW1, an antibody against polyQ, reveals that polyQ adopts an, extended, coil-like structure. Consistent with the linear lattice model, multimeric MW1 Fvs bind more tightly to longer than to shorter polyQ, tracts and, compared with monomeric Fv, bind expanded polyQ repeats with, higher apparent affinities. These results suggest a mechanism for the, toxicity of expanded polyQ and a strategy to link anti-polyQ compounds to, create high-avidity therapeutics.
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<StructureSection load='2gsg' size='340' side='right'caption='[[2gsg]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gsg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GSG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gsg OCA], [https://pdbe.org/2gsg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gsg RCSB], [https://www.ebi.ac.uk/pdbsum/2gsg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gsg ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/2gsg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gsg ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Huntington and related neurological diseases result from expansion of a polyglutamine (polyQ) tract. The linear lattice model for the structure and binding properties of polyQ proposes that both expanded and normal polyQ tracts in the preaggregation state are random-coil structures but that an expanded polyQ repeat contains a larger number of epitopes recognized by antibodies or other proteins. The crystal structure of polyQ bound to MW1, an antibody against polyQ, reveals that polyQ adopts an extended, coil-like structure. Consistent with the linear lattice model, multimeric MW1 Fvs bind more tightly to longer than to shorter polyQ tracts and, compared with monomeric Fv, bind expanded polyQ repeats with higher apparent affinities. These results suggest a mechanism for the toxicity of expanded polyQ and a strategy to link anti-polyQ compounds to create high-avidity therapeutics.
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==About this Structure==
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The structure of a polyQ-anti-polyQ complex reveals binding according to a linear lattice model.,Li P, Huey-Tubman KE, Gao T, Li X, West AP Jr, Bennett MJ, Bjorkman PJ Nat Struct Mol Biol. 2007 May;14(5):381-7. Epub 2007 Apr 22. PMID:17450152<ref>PMID:17450152</ref>
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2GSG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GSG OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The structure of a polyQ-anti-polyQ complex reveals binding according to a linear lattice model., Li P, Huey-Tubman KE, Gao T, Li X, West AP Jr, Bennett MJ, Bjorkman PJ, Nat Struct Mol Biol. 2007 May;14(5):381-7. Epub 2007 Apr 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17450152 17450152]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 2gsg" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Bennett, M.J.]]
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[[Category: Bjorkman, P.J.]]
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[[Category: Huey-Tubman, K.E.]]
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[[Category: Jr., A.P.West.]]
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[[Category: Li, P.]]
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[[Category: SO4]]
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[[Category: fv]]
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[[Category: monoclonal antibody]]
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[[Category: poly-glutamine]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:50:25 2007''
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Bennett MJ]]
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[[Category: Bjorkman PJ]]
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[[Category: Huey-Tubman KE]]
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[[Category: Li P]]
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[[Category: West Jr AP]]

Current revision

Crystal structure of the Fv fragment of a monoclonal antibody specific for poly-glutamine

PDB ID 2gsg

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