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| - | [[Image:2qow.gif|left|200px]] | + | #REDIRECT [[4v56]] This PDB entry is obsolete and replaced by 4v56 |
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| - | {{STRUCTURE_2qow| PDB=2qow | SCENE= }}
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| - | '''Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the second 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.'''
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| - | ==Overview==
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| - | The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.
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| - | ==About this Structure==
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| - | 2QOW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOW OCA].
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| - | ==Reference==
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| - | A steric block in translation caused by the antibiotic spectinomycin., Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH, ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17696316 17696316]
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Protein complex]]
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| - | [[Category: Salmonella typhimurium]]
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| - | [[Category: Borovinskaya, M A.]]
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| - | [[Category: Cate, J H.D.]]
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| - | [[Category: Fredrick, K.]]
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| - | [[Category: Holton, J M.]]
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| - | [[Category: Shoji, S.]]
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| - | [[Category: Acetylation]]
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| - | [[Category: Antibiotic resistance]]
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| - | [[Category: Endonuclease]]
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| - | [[Category: Hydrolase]]
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| - | [[Category: Methylation]]
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| - | [[Category: Nuclease]]
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| - | [[Category: Repressor]]
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| - | [[Category: Ribonucleoprotein]]
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| - | [[Category: Ribosomal protein]]
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| - | [[Category: Ribosome]]
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| - | [[Category: Rna-binding]]
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| - | [[Category: Rna-protein complex]]
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| - | [[Category: Rrna-binding]]
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| - | [[Category: Transcription]]
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| - | [[Category: Transcription regulation]]
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| - | [[Category: Transcription termination]]
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| - | [[Category: Translation regulation]]
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| - | [[Category: Trna-binding]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:20:38 2008''
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