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2r6g

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[[Image:2r6g.jpg|left|200px]]
 
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==The Crystal Structure of the E. coli Maltose Transporter==
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The line below this paragraph, containing "STRUCTURE_2r6g", creates the "Structure Box" on the page.
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<StructureSection load='2r6g' size='340' side='right'caption='[[2r6g]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2r6g]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R6G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
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{{STRUCTURE_2r6g| PDB=2r6g | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r6g OCA], [https://pdbe.org/2r6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r6g RCSB], [https://www.ebi.ac.uk/pdbsum/2r6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r6g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MALK_ECOLI MALK_ECOLI] Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Responsible for energy coupling to the transport system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r6/2r6g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r6g ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The maltose uptake system of Escherichia coli is a well-characterized member of the ATP-binding cassette transporter superfamily. Here we present the 2.8-A crystal structure of the intact maltose transporter in complex with the maltose-binding protein, maltose and ATP. This structure, stabilized by a mutation that prevents ATP hydrolysis, captures the ATP-binding cassette dimer in a closed, ATP-bound conformation. Maltose is occluded within a solvent-filled cavity at the interface of the two transmembrane subunits, about halfway into the lipid bilayer. The binding protein docks onto the entrance of the cavity in an open conformation and serves as a cap to ensure unidirectional translocation of the sugar molecule. These results provide direct evidence for a concerted mechanism of transport in which solute is transferred from the binding protein to the transmembrane subunits when the cassette dimer closes to hydrolyse ATP.
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'''The Crystal Structure of the E. coli Maltose Transporter'''
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Crystal structure of a catalytic intermediate of the maltose transporter.,Oldham ML, Khare D, Quiocho FA, Davidson AL, Chen J Nature. 2007 Nov 22;450(7169):515-21. PMID:18033289<ref>PMID:18033289</ref>
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==Overview==
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The maltose uptake system of Escherichia coli is a well-characterized member of the ATP-binding cassette transporter superfamily. Here we present the 2.8-A crystal structure of the intact maltose transporter in complex with the maltose-binding protein, maltose and ATP. This structure, stabilized by a mutation that prevents ATP hydrolysis, captures the ATP-binding cassette dimer in a closed, ATP-bound conformation. Maltose is occluded within a solvent-filled cavity at the interface of the two transmembrane subunits, about halfway into the lipid bilayer. The binding protein docks onto the entrance of the cavity in an open conformation and serves as a cap to ensure unidirectional translocation of the sugar molecule. These results provide direct evidence for a concerted mechanism of transport in which solute is transferred from the binding protein to the transmembrane subunits when the cassette dimer closes to hydrolyse ATP.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2R6G is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R6G OCA].
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</div>
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<div class="pdbe-citations 2r6g" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Crystal structure of a catalytic intermediate of the maltose transporter., Oldham ML, Khare D, Quiocho FA, Davidson AL, Chen J, Nature. 2007 Nov 22;450(7169):515-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18033289 18033289]
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*[[Maltose-binding protein 3D structures|Maltose-binding protein 3D structures]]
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[[Category: Escherichia coli]]
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== References ==
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[[Category: Maltose-transporting ATPase]]
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<references/>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Chen, J.]]
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</StructureSection>
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[[Category: Davidson, A L.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Khare, D.]]
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[[Category: Large Structures]]
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[[Category: Oldham, M L]]
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[[Category: Chen J]]
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[[Category: Quiocho, F A.]]
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[[Category: Davidson AL]]
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[[Category: Abc transporter]]
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[[Category: Khare D]]
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[[Category: Atp binding cassette]]
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[[Category: Oldham ML]]
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[[Category: Atp-binding]]
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[[Category: Quiocho FA]]
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[[Category: Catalytic intermediate]]
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[[Category: E. coli maltose transporter]]
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[[Category: Hydrolase]]
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[[Category: Hydrolase/transport protein complex]]
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[[Category: Inner membrane]]
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[[Category: Malk]]
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[[Category: Maltodextrin binding protein]]
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[[Category: Mbp]]
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[[Category: Membrane]]
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[[Category: Nucleotide-binding]]
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[[Category: Periplasm]]
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[[Category: Sugar transport]]
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[[Category: Transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:19:32 2008''
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Current revision

The Crystal Structure of the E. coli Maltose Transporter

PDB ID 2r6g

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