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194l

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(New page: 200px<br /><applet load="194l" size="450" color="white" frame="true" align="right" spinBox="true" caption="194l, resolution 1.40&Aring;" /> '''THE 1.40 A STRUCTURE...)
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[[Image:194l.gif|left|200px]]<br /><applet load="194l" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="194l, resolution 1.40&Aring;" />
 
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'''THE 1.40 A STRUCTURE OF SPACEHAB-01 HEN EGG WHITE LYSOZYME'''<br />
 
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==Overview==
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==THE 1.40 A STRUCTURE OF SPACEHAB-01 HEN EGG WHITE LYSOZYME==
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Crystals of tetragonal hen egg-white lysozyme were grown using Advanced, Protein Crystallization Facility (APCF) apparatus under a microgravity, environment (SpaceHab-01 mission) and ground control conditions. Crystals, were grown from NaCl as a crystallizing agent at pH 4.3. The X-ray, diffraction patterns of the best diffracting ground- and space-grown, crystals were recorded using synchrotron radiation and an image plate on, the W32 beamline at LURE. Both ground- and space-grown crystals showed, nearly equivalent maximum resolution of 1.3-1.4 A. Refinements were, carried out with the program X-PLOR with final R values of 18.45 and, 18.27% for structures from ground- and space- grown crystals, respectively. The two structures are nearly identical with the, root-mean-square difference on all protein atoms being 0.13 A. Some, residues of the two refined structures show multiple alternative, conformations. Two ions were localized into the electron-density maps of, the two structures: one chloride ion at the interface between two, symmetry-related molecules and one sodium ion stabilizing the loop, Ser60-Leu75. The sodium ion is surrounded by six ligands which form a, bipyramid around it at distances of 2.2-2.6 A.
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<StructureSection load='194l' size='340' side='right'caption='[[194l]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[194l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=194L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=194L FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=194l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=194l OCA], [https://pdbe.org/194l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=194l RCSB], [https://www.ebi.ac.uk/pdbsum/194l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=194l ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/94/194l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=194l ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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194L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with CL and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=194L OCA].
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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== References ==
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==Reference==
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<references/>
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High-resolution structure (1.33 A) of a HEW lysozyme tetragonal crystal grown in the APCF apparatus. Data and structural comparison with a crystal grown under microgravity from SpaceHab-01 mission., Vaney MC, Maignan S, Ries-Kautt M, Ducriux A, Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):505-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299672 15299672]
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__TOC__
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</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ducruix, A.]]
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[[Category: Ducruix A]]
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[[Category: Maignan, S.]]
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[[Category: Maignan S]]
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[[Category: Ries-Kautt, M.]]
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[[Category: Ries-Kautt M]]
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[[Category: Vaney, M.C.]]
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[[Category: Vaney MC]]
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[[Category: CL]]
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[[Category: NA]]
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[[Category: hydrolase (o-glycosyl)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:31:03 2007''
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Current revision

THE 1.40 A STRUCTURE OF SPACEHAB-01 HEN EGG WHITE LYSOZYME

PDB ID 194l

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