2v26

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[[Image:2v26.jpg|left|200px]]
 
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==Myosin VI (MD) pre-powerstroke state (Mg.ADP.VO4)==
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The line below this paragraph, containing "STRUCTURE_2v26", creates the "Structure Box" on the page.
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<StructureSection load='2v26' size='340' side='right'caption='[[2v26]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2v26]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V26 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
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{{STRUCTURE_2v26| PDB=2v26 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v26 OCA], [https://pdbe.org/2v26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v26 RCSB], [https://www.ebi.ac.uk/pdbsum/2v26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v26 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYO6_PIG MYO6_PIG] Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. Required for structural integrity of inner ear hair cells (By similarity).<ref>PMID:16917816</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v2/2v26_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v26 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Due to a unique addition to the lever arm-positioning region (converter), class VI myosins move in the opposite direction (toward the minus-end of actin filaments) compared to other characterized myosin classes. However, the large size of the myosin VI lever arm swing (powerstroke) cannot be explained by our current view of the structural transitions that occur within the myosin motor. We have solved the crystal structure of a fragment of the myosin VI motor in the structural state that represents the starting point for movement on actin; the pre-powerstroke state. Unexpectedly, the converter itself rearranges to achieve a conformation that has not been seen for other myosins. This results in a much larger powerstroke than is achievable without the converter rearrangement. Moreover, it provides a new mechanism that could be exploited to increase the powerstroke of yet to be characterized plus-end-directed myosin classes.
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'''MYOSIN VI (MD) PRE-POWERSTROKE STATE (MG.ADP.VO4)'''
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The structural basis for the large powerstroke of myosin VI.,Menetrey J, Llinas P, Mukherjea M, Sweeney HL, Houdusse A Cell. 2007 Oct 19;131(2):300-8. PMID:17956731<ref>PMID:17956731</ref>
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==Overview==
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Due to a unique addition to the lever arm-positioning region (converter), class VI myosins move in the opposite direction (toward the minus-end of actin filaments) compared to other characterized myosin classes. However, the large size of the myosin VI lever arm swing (powerstroke) cannot be explained by our current view of the structural transitions that occur within the myosin motor. We have solved the crystal structure of a fragment of the myosin VI motor in the structural state that represents the starting point for movement on actin; the pre-powerstroke state. Unexpectedly, the converter itself rearranges to achieve a conformation that has not been seen for other myosins. This results in a much larger powerstroke than is achievable without the converter rearrangement. Moreover, it provides a new mechanism that could be exploited to increase the powerstroke of yet to be characterized plus-end-directed myosin classes.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2V26 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V26 OCA].
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</div>
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<div class="pdbe-citations 2v26" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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The structural basis for the large powerstroke of myosin VI., Menetrey J, Llinas P, Mukherjea M, Sweeney HL, Houdusse A, Cell. 2007 Oct 19;131(2):300-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17956731 17956731]
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*[[Myosin 3D Structures|Myosin 3D Structures]]
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[[Category: Single protein]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Houdusse, A.]]
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[[Category: Houdusse A]]
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[[Category: Llinas, P.]]
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[[Category: Llinas P]]
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[[Category: Menetrey, J.]]
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[[Category: Menetrey J]]
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[[Category: Mukherjea, M.]]
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[[Category: Mukherjea M]]
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[[Category: Sweeney, H L.]]
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[[Category: Sweeney HL]]
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[[Category: Actin-binding]]
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[[Category: Atp-binding]]
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[[Category: Calmodulin-binding]]
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[[Category: Coiled coil]]
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[[Category: Domain motor]]
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[[Category: Endocytosis]]
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[[Category: Golgi apparatus]]
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[[Category: Membrane]]
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[[Category: Molecular motor]]
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[[Category: Motor protein]]
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[[Category: Myosin]]
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[[Category: Myosin vi]]
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[[Category: Nuclear protein]]
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[[Category: Nucleotide-binding]]
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[[Category: Phosphorylation]]
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[[Category: Pre- powerstroke]]
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[[Category: Protein transport]]
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[[Category: Structural protein]]
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[[Category: Transition state]]
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[[Category: Transport]]
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[[Category: Vanadate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:05:03 2008''
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Current revision

Myosin VI (MD) pre-powerstroke state (Mg.ADP.VO4)

PDB ID 2v26

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