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| - | [[Image:2v75.jpg|left|200px]] | |
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| - | <!--
| + | ==N-terminal domain of Nab2== |
| - | The line below this paragraph, containing "STRUCTURE_2v75", creates the "Structure Box" on the page.
| + | <StructureSection load='2v75' size='340' side='right'caption='[[2v75]], [[Resolution|resolution]] 1.80Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[2v75]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V75 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
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| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v75 OCA], [https://pdbe.org/2v75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v75 RCSB], [https://www.ebi.ac.uk/pdbsum/2v75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v75 ProSAT]</span></td></tr> |
| - | {{STRUCTURE_2v75| PDB=2v75 | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | '''N-TERMINAL DOMAIN OF NAB2''' | + | [https://www.uniprot.org/uniprot/NAB2_YEAST NAB2_YEAST] This essential protein binds to polyadenylated RNA and single-stranded DNA. It may be involved not only in RNA processing but also in transcription regulation. Believed to associate directly with nascent RNA polymerase II transcripts and remain associated during subsequent nuclear RNA processing reactions. |
| - | | + | __TOC__ |
| - | | + | </StructureSection> |
| - | ==Overview== | + | [[Category: Large Structures]] |
| - | Nuclear abundant poly(A) RNA-binding protein 2 (Nab2) is an essential yeast heterogeneous nuclear ribonucleoprotein that modulates both mRNA nuclear export and poly(A) tail length. The N-terminal domain of Nab2 (residues 1-97) mediates interactions with both the C-terminal globular domain of the nuclear pore-associated protein, myosin-like protein 1 (Mlp1), and the mRNA export factor, Gfd1. The solution and crystal structures of the Nab2 N-terminal domain show a primarily helical fold that is analogous to the PWI fold found in several other RNA-binding proteins. In contrast to other PWI-containing proteins, we find no evidence that the Nab2 N-terminal domain binds to nucleic acids. Instead, this domain appears to mediate protein:protein interactions that facilitate the nuclear export of mRNA. The Nab2 N-terminal domain has a distinctive hydrophobic patch centered on Phe73, consistent with this region of the surface being a protein:protein interaction site. Engineered mutations within this hydrophobic patch attenuate the interaction with the Mlp1 C-terminal domain but do not alter the interaction with Gfd1, indicating that this patch forms a crucial component of the interface between Nab2 and Mlp1.
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| - | ==About this Structure== | + | |
| - | 2V75 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V75 OCA].
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| - | | + | |
| - | ==Reference== | + | |
| - | Structure of the N-Terminal Mlp1-Binding Domain of the Saccharomyces cerevisiae mRNA-Binding Protein, Nab2., Grant RP, Marshall NJ, Yang JC, Fasken MB, Kelly SM, Harreman MT, Neuhaus D, Corbett AH, Stewart M, J Mol Biol. 2007 Dec 4;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18190927 18190927]
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| | [[Category: Saccharomyces cerevisiae]] | | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Single protein]]
| + | [[Category: Grant RP]] |
| - | [[Category: Grant, R P.]] | + | [[Category: Marshall NJ]] |
| - | [[Category: Marshall, N J.]] | + | [[Category: Stewart M]] |
| - | [[Category: Stewart, M.]] | + | |
| - | [[Category: Metal-binding]]
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| - | [[Category: Nuclear protein]]
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| - | [[Category: Nucleus]]
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| - | [[Category: Rna-binding]]
| + | |
| - | [[Category: Zinc]]
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| - | [[Category: Zinc-finger]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:18:38 2008''
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