1a70

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(New page: 200px<br /><applet load="1a70" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a70, resolution 1.7&Aring;" /> '''SPINACH FERREDOXIN'''...)
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[[Image:1a70.gif|left|200px]]<br /><applet load="1a70" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a70, resolution 1.7&Aring;" />
 
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'''SPINACH FERREDOXIN'''<br />
 
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==Overview==
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==SPINACH FERREDOXIN==
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Ferredoxin I (Fd I) from Spinacia oleracea is composed of 97 amino-acid, residues and a [2Fe-2S] cluster. The crystal structure of the E92K mutant, of Fd I was solved by molecular replacement and refined to an R factor of, 19.6% for 11755 reflections at 1.7 A resolution. The overall structure and, the active centre of spinach Fd is highly conserved with respect to, ferredoxins of known structure. The E92K mutation appears to disturb a, hydrogen-bond network which stabilizes the loop bearing the [2Fe-2S], cluster. This observation provides a rationale for the reduced, electron-transfer efficiency displayed by the E92K mutant. Inspection of, the crystal packing reveals that the side chain of Lys92 is engaged in an, intermolecular interaction with Asp26 of a symmetry-related molecule. This, feature may explain why only the mutant E92K and not wild-type Fd I could, be successfully crystallized.
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<StructureSection load='1a70' size='340' side='right'caption='[[1a70]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1a70]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A70 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a70 OCA], [https://pdbe.org/1a70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a70 RCSB], [https://www.ebi.ac.uk/pdbsum/1a70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a70 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FER1_SPIOL FER1_SPIOL] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/1a70_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a70 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ferredoxin I (Fd I) from Spinacia oleracea is composed of 97 amino-acid residues and a [2Fe-2S] cluster. The crystal structure of the E92K mutant of Fd I was solved by molecular replacement and refined to an R factor of 19.6% for 11755 reflections at 1.7 A resolution. The overall structure and the active centre of spinach Fd is highly conserved with respect to ferredoxins of known structure. The E92K mutation appears to disturb a hydrogen-bond network which stabilizes the loop bearing the [2Fe-2S] cluster. This observation provides a rationale for the reduced electron-transfer efficiency displayed by the E92K mutant. Inspection of the crystal packing reveals that the side chain of Lys92 is engaged in an intermolecular interaction with Asp26 of a symmetry-related molecule. This feature may explain why only the mutant E92K and not wild-type Fd I could be successfully crystallized.
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==About this Structure==
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Structure of the mutant E92K of [2Fe-2S] ferredoxin I from Spinacia oleracea at 1.7 A resolution.,Binda C, Coda A, Aliverti A, Zanetti G, Mattevi A Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1353-8. PMID:10089511<ref>PMID:10089511</ref>
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1A70 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea] with FES as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A70 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of the mutant E92K of [2Fe-2S] ferredoxin I from Spinacia oleracea at 1.7 A resolution., Binda C, Coda A, Aliverti A, Zanetti G, Mattevi A, Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1353-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089511 10089511]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1a70" style="background-color:#fffaf0;"></div>
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[[Category: Spinacia oleracea]]
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[[Category: Aliverti, A.]]
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[[Category: Binda, C.]]
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[[Category: Coda, A.]]
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[[Category: Mattevi, A.]]
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[[Category: Zanetti, G.]]
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[[Category: FES]]
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[[Category: electron transport]]
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[[Category: iron-sulfur protein]]
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[[Category: photosynthesis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:39:02 2007''
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==See Also==
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*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Spinacia oleracea]]
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[[Category: Aliverti A]]
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[[Category: Binda C]]
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[[Category: Coda A]]
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[[Category: Mattevi A]]
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[[Category: Zanetti G]]

Current revision

SPINACH FERREDOXIN

PDB ID 1a70

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