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1a7d

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(New page: 200px<br /><applet load="1a7d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a7d, resolution 1.8&Aring;" /> '''CHLOROMET MYOHEMERYTH...)
Current revision (06:28, 7 February 2024) (edit) (undo)
 
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[[Image:1a7d.gif|left|200px]]<br /><applet load="1a7d" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a7d, resolution 1.8&Aring;" />
 
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'''CHLOROMET MYOHEMERYTHRIN FROM THEMISTE ZOSTERICOLA'''<br />
 
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==Overview==
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==CHLOROMET MYOHEMERYTHRIN FROM THEMISTE ZOSTERICOLA==
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Myohemerythrin (Mhr) is a nonheme iron oxygen carrier found in the, retractor muscles of marine "peanut" worms. The X-ray crystal structures, of two recombinant Themiste zostericola Mhrs are reported to a resolution, of 1.8 A. Surprisingly, the met wild-type structure (R = 17.8%) was found, to contain chloride bound to Fe2, while coordinated hydroxide was found in, the met L103N structure (R = 18.3%). An internal water molecule was also, found distal to the Fe-O-Fe center of the mutant protein, forming hydrogen, bonds with the coordinated hydroxide and the OD1 atom of Asn-103. This, finding is consistent with the kinetic and spectroscopic results reported, for the L103N mutant Mhr [Raner, G. M., Martins, L. J., &amp; Ellis, W. R., Jr. (1997) Biochemistry 36, 7037-7043]. Possible roles for the side chain, of residue 103 (Leu in wild-type Mhr) in gating ligand binding are also, discussed.
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<StructureSection load='1a7d' size='340' side='right'caption='[[1a7d]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1a7d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Themiste_hennahi Themiste hennahi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A7D FirstGlance]. <br>
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1A7D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Themiste_zostericola Themiste zostericola] with CL and CFO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A7D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CFO:CHLORO+DIIRON-OXO+MOIETY'>CFO</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a7d OCA], [https://pdbe.org/1a7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a7d RCSB], [https://www.ebi.ac.uk/pdbsum/1a7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a7d ProSAT]</span></td></tr>
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Structures of wild-type chloromet and L103N hydroxomet Themiste zostericola myohemerythrins at 1.8 A resolution., Martins LJ, Hill CP, Ellis WR Jr, Biochemistry. 1997 Jun 10;36(23):7044-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9188702 9188702]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Themiste zostericola]]
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[https://www.uniprot.org/uniprot/HEMTM_THEHE HEMTM_THEHE] Myohemerythrin is an oxygen-binding protein found in the retractor muscles of certain worms. The oxygen-binding site contains two iron atoms.
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[[Category: Hill, C.P.]]
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== Evolutionary Conservation ==
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[[Category: Junior, W.R.Ellis.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Martins, L.J.]]
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Check<jmol>
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[[Category: CFO]]
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<jmolCheckbox>
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[[Category: CL]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/1a7d_consurf.spt"</scriptWhenChecked>
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[[Category: nonheme iron oxygen carrier]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: oxygen transport]]
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:39:45 2007''
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a7d ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Themiste hennahi]]
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[[Category: Ellis Junior WR]]
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[[Category: Hill CP]]
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[[Category: Martins LJ]]

Current revision

CHLOROMET MYOHEMERYTHRIN FROM THEMISTE ZOSTERICOLA

PDB ID 1a7d

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