1a8h

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(New page: 200px<br /><applet load="1a8h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a8h, resolution 2.00&Aring;" /> '''METHIONYL-TRNA SYNTH...)
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[[Image:1a8h.gif|left|200px]]<br /><applet load="1a8h" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a8h, resolution 2.00&Aring;" />
 
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'''METHIONYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS'''<br />
 
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==Overview==
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==METHIONYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS==
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Background: The 20 aminoacyl-tRNA synthetases are divided into two, classes, I and II. The 10 class I synthetases are considered to have in, common the catalytic domain structure based on the Rossmann fold, which is, totally different from the class II catalytic domain structure. The class, I synthetases are further divided into three subclasses, a, b and c, according to sequence homology. No conserved structural features for tRNA, recognition by class I synthetases have been established. Results: We, determined the crystal structure of the class Ia methionyl-tRNA synthetase, (MetRS) at 2.0 A resolution, using MetRS from an extreme thermophile, Thermus thermophilus HB8. The T. thermophilus MetRS structure is in full, agreement with the biochemical and genetic data from Escherichia coli, MetRS. The conserved 'anticodon-binding' residues are spatially clustered, on an alpha-helix-bundle domain. The Rossmann-fold and anticodon-binding, domains are connected by a beta-alpha-alpha-beta-alpha topology ('SC, fold') domain that contains the class I specific KMSKS motif. Conclusions:, The alpha-helix-bundle domain identified in the MetRS structure is the, signature of the class Ia enzymes, as it was also identified in the class, Ia structures of the isoleucyl- and arginyl-tRNA synthetases. The, beta-alpha-alpha-beta-alpha topology domain, which can now be identified, in all known structures of the class Ia and Ib synthetases, is likely to, dock with the inner side of the L-shaped tRNA, thereby positioning the, anticodon stem.
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<StructureSection load='1a8h' size='340' side='right'caption='[[1a8h]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1a8h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A8H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a8h OCA], [https://pdbe.org/1a8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a8h RCSB], [https://www.ebi.ac.uk/pdbsum/1a8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a8h ProSAT], [https://www.topsan.org/Proteins/RSGI/1a8h TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYM_THET8 SYM_THET8] Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/1a8h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a8h ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1A8H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Methionine--tRNA_ligase Methionine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.10 6.1.1.10] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A8H OCA].
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The 2.0 A crystal structure of Thermus thermophilus methionyl-tRNA synthetase reveals two RNA-binding modules., Sugiura I, Nureki O, Ugaji-Yoshikawa Y, Kuwabara S, Shimada A, Tateno M, Lorber B, Giege R, Moras D, Yokoyama S, Konno M, Structure. 2000 Feb 15;8(2):197-208. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10673435 10673435]
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[[Category: Large Structures]]
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[[Category: Methionine--tRNA ligase]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Single protein]]
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[[Category: Giege R]]
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[[Category: Thermus thermophilus]]
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[[Category: Konno M]]
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[[Category: Giege, R.]]
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[[Category: Kuwabara S]]
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[[Category: Konno, M.]]
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[[Category: Lober B]]
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[[Category: Kuwabara, S.]]
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[[Category: Moras D]]
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[[Category: Lober, B.]]
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[[Category: Nureki O]]
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[[Category: Moras, D.]]
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[[Category: Sugiura I]]
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[[Category: Nureki, O.]]
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[[Category: Ugaji Y]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Yokoyama S]]
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[[Category: Sugiura, I.]]
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[[Category: Ugaji, Y.]]
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[[Category: Yokoyama, S.]]
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[[Category: ZN]]
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[[Category: aminoacyl-trna synthetase]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rossmann fold]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:40:47 2007''
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Current revision

METHIONYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS

PDB ID 1a8h

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