This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1a8z

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1a8z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a8z, resolution 2.1&Aring;" /> '''STRUCTURE DETERMINATI...)
Current revision (06:28, 7 February 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1a8z.gif|left|200px]]<br /><applet load="1a8z" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1a8z, resolution 2.1&Aring;" />
 
-
'''STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS'''<br />
 
-
==Overview==
+
==STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS==
-
The structure of rusticyanin, an acid-stable copper protein, has been, determined at 2.1 A resolution by direct methods combined with the, single-wavelength anomalous scattering (SAS) of copper (f" = 3.9 e-) and, then conventionally refined (Rcryst = 18.7%, Rfree = 21.9%). This is the, largest unknown protein structure (Mr approximately /= 16.8 kDa) to be, determined using the SAS and direct-methods approach and demonstrates that, by exploiting the anomalous signal at a single wavelength, direct methods, can be used to determine phases at typical (approximately 2 A), macromolecular crystallographic resolutions. Extrapolating from the size, of the anomalous signal for copper (f" approximately 4 e-), this result, suggests that the approach could be used for proteins with molecular, weights of up to 33 kDa per Se (f"max++ = 8 e- at the 'white line') and 80, kDa for a Pt derivative (f"max = 19 e- at the 'white line', L3 edge). The, method provides a powerful alternative in solving a de novo protein, structure without either preparing multiple crystals (i.e. isomorphous, heavy-atom derivative plus native crystals) or collecting multi-wavelength, anomalous diffraction (MAD) data.
+
<StructureSection load='1a8z' size='340' side='right'caption='[[1a8z]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1a8z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A8Z FirstGlance]. <br>
-
1A8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans] with CU1 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A8Z OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a8z OCA], [https://pdbe.org/1a8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a8z RCSB], [https://www.ebi.ac.uk/pdbsum/1a8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a8z ProSAT]</span></td></tr>
-
Structure determination of a 16.8 kDa copper protein at 2.1 A resolution using anomalous scattering data with direct methods., Harvey I, Hao Q, Duke EM, Ingledew WJ, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):629-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9761859 9761859]
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RUS2_ACIFI RUS2_ACIFI] Electron carrier from cytochrome c552 to the A-type oxidase.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/1a8z_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a8z ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Acidithiobacillus ferrooxidans]]
[[Category: Acidithiobacillus ferrooxidans]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Duke, E.M.H.]]
+
[[Category: Duke EMH]]
-
[[Category: Hao, Q.]]
+
[[Category: Hao Q]]
-
[[Category: Harvey, I.]]
+
[[Category: Harvey I]]
-
[[Category: Hasnain, S.S.]]
+
[[Category: Hasnain SS]]
-
[[Category: Ingledew, W.J.]]
+
[[Category: Ingledew WJ]]
-
[[Category: CU1]]
+
-
[[Category: copper protein]]
+
-
[[Category: direct methods]]
+
-
[[Category: electron transport]]
+
-
[[Category: medium resolution]]
+
-
[[Category: metalloprotein]]
+
-
[[Category: sas]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:41:20 2007''
+

Current revision

STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS

PDB ID 1a8z

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1a8z"
Personal tools