1a8z

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(New page: 200px<br /><applet load="1a8z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a8z, resolution 2.1&Aring;" /> '''STRUCTURE DETERMINATI...)
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[[Image:1a8z.gif|left|200px]]<br /><applet load="1a8z" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a8z, resolution 2.1&Aring;" />
 
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'''STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS'''<br />
 
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==Overview==
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==STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS==
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The structure of rusticyanin, an acid-stable copper protein, has been, determined at 2.1 A resolution by direct methods combined with the, single-wavelength anomalous scattering (SAS) of copper (f" = 3.9 e-) and, then conventionally refined (Rcryst = 18.7%, Rfree = 21.9%). This is the, largest unknown protein structure (Mr approximately /= 16.8 kDa) to be, determined using the SAS and direct-methods approach and demonstrates that, by exploiting the anomalous signal at a single wavelength, direct methods, can be used to determine phases at typical (approximately 2 A), macromolecular crystallographic resolutions. Extrapolating from the size, of the anomalous signal for copper (f" approximately 4 e-), this result, suggests that the approach could be used for proteins with molecular, weights of up to 33 kDa per Se (f"max++ = 8 e- at the 'white line') and 80, kDa for a Pt derivative (f"max = 19 e- at the 'white line', L3 edge). The, method provides a powerful alternative in solving a de novo protein, structure without either preparing multiple crystals (i.e. isomorphous, heavy-atom derivative plus native crystals) or collecting multi-wavelength, anomalous diffraction (MAD) data.
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<StructureSection load='1a8z' size='340' side='right'caption='[[1a8z]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1a8z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A8Z FirstGlance]. <br>
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1A8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans] with CU1 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A8Z OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a8z OCA], [https://pdbe.org/1a8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a8z RCSB], [https://www.ebi.ac.uk/pdbsum/1a8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a8z ProSAT]</span></td></tr>
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Structure determination of a 16.8 kDa copper protein at 2.1 A resolution using anomalous scattering data with direct methods., Harvey I, Hao Q, Duke EM, Ingledew WJ, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):629-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9761859 9761859]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RUS2_ACIFI RUS2_ACIFI] Electron carrier from cytochrome c552 to the A-type oxidase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/1a8z_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a8z ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Acidithiobacillus ferrooxidans]]
[[Category: Acidithiobacillus ferrooxidans]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Duke, E.M.H.]]
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[[Category: Duke EMH]]
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[[Category: Hao, Q.]]
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[[Category: Hao Q]]
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[[Category: Harvey, I.]]
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[[Category: Harvey I]]
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[[Category: Hasnain, S.S.]]
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[[Category: Hasnain SS]]
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[[Category: Ingledew, W.J.]]
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[[Category: Ingledew WJ]]
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[[Category: CU1]]
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[[Category: copper protein]]
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[[Category: direct methods]]
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[[Category: electron transport]]
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[[Category: medium resolution]]
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[[Category: metalloprotein]]
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[[Category: sas]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:41:20 2007''
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Current revision

STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS

PDB ID 1a8z

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