1ae9

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(New page: 200px<br /><applet load="1ae9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ae9, resolution 1.9&Aring;" /> '''STRUCTURE OF THE LAMB...)
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[[Image:1ae9.gif|left|200px]]<br /><applet load="1ae9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ae9, resolution 1.9&Aring;" />
 
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'''STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE'''<br />
 
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==Overview==
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==STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE==
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Lambda integrase is archetypic of site-specific recombinases that catalyze, intermolecular DNA rearrangements without energetic input. DNA cleavage, strand exchange, and religation steps are linked by a covalent, phosphotyrosine intermediate in which Tyr342 is attached to the, 3'-phosphate of the DNA cut site. The 1.9 angstrom crystal structure of, the integrase catalytic domain reveals a protein fold that is conserved in, organisms ranging from archaebacteria to yeast and that suggests a model, for interaction with target DNA. The attacking Tyr342 nucleophile is, located on a flexible loop about 20 angstroms from a basic groove that, contains all the other catalytically essential residues. This bipartite, active site can account for several apparently paradoxical features of, integrase family recombinases, including the capacity for both cis and, trans cleavage of DNA.
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<StructureSection load='1ae9' size='340' side='right'caption='[[1ae9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae9 OCA], [https://pdbe.org/1ae9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VINT_LAMBD VINT_LAMBD] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae9 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AE9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA].
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*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Flexibility in DNA recombination: structure of the lambda integrase catalytic core., Kwon HJ, Tirumalai R, Landy A, Ellenberger T, Science. 1997 Apr 4;276(5309):126-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9082984 9082984]
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[[Category: Escherichia virus Lambda]]
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[[Category: Enterobacteria phage lambda]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Ellenberger T]]
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[[Category: Ellenberger, T.]]
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[[Category: Kwon HJ]]
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[[Category: Kwon, H.J.]]
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[[Category: Landy A]]
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[[Category: Landy, A.]]
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[[Category: Tirumalai R]]
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[[Category: Tirumalai, R.]]
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[[Category: dna recombination]]
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[[Category: integrase]]
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[[Category: site-specific recombination]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:47:31 2007''
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Current revision

STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE

PDB ID 1ae9

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