1af5

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(New page: 200px<br /><applet load="1af5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1af5, resolution 3.00&Aring;" /> '''GROUP I MOBILE INTRO...)
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[[Image:1af5.gif|left|200px]]<br /><applet load="1af5" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1af5, resolution 3.00&Aring;" />
 
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'''GROUP I MOBILE INTRON ENDONUCLEASE'''<br />
 
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==Overview==
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==GROUP I MOBILE INTRON ENDONUCLEASE==
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The structure of I-Crel provides the first view of a protein encoded by a, gene within an intron. This endonuclease recognizes a long DNA site, approximately 20 base pairs in length and facilitates the lateral transfer, of that intron. The protein exhibits a DNA-binding surface consisting of, four antiparallel beta-strands that form a 20 A wide groove which is over, 70 A long. The architecture of this fold is different from that of the, TATA binding protein, TBP, which also contains an antiparallel, beta-saddle. The conserved LAGLIDADG motif, which is found in many mobile, intron endonucleases, maturases and inteins, forms a novel helical, interface and contributes essential residues to the active site.
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<StructureSection load='1af5' size='340' side='right'caption='[[1af5]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1af5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AF5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1af5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af5 OCA], [https://pdbe.org/1af5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1af5 RCSB], [https://www.ebi.ac.uk/pdbsum/1af5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1af5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/1af5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1af5 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AF5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AF5 OCA].
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The structure of I-Crel, a group I intron-encoded homing endonuclease., Heath PJ, Stephens KM, Monnat RJ Jr, Stoddard BL, Nat Struct Biol. 1997 Jun;4(6):468-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9187655 9187655]
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[[Category: Chlamydomonas reinhardtii]]
[[Category: Chlamydomonas reinhardtii]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Heath, P.J.]]
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[[Category: Heath PJ]]
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[[Category: Junior, R.J.Monnat.]]
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[[Category: Monnat Junior RJ]]
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[[Category: Stephens, K.M.]]
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[[Category: Stephens KM]]
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[[Category: Stoddard, B.L.]]
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[[Category: Stoddard BL]]
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[[Category: chloroplast dna]]
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[[Category: endonuclease]]
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[[Category: group i mobile intron]]
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[[Category: intron homing]]
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[[Category: laglidadg motif]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:48:23 2007''
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Current revision

GROUP I MOBILE INTRON ENDONUCLEASE

PDB ID 1af5

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