2z1t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:55, 13 March 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2z1t.gif|left|200px]]
 
-
<!--
+
==Crystal Structure of Hydrogenase Maturation Protein HypE==
-
The line below this paragraph, containing "STRUCTURE_2z1t", creates the "Structure Box" on the page.
+
<StructureSection load='2z1t' size='340' side='right'caption='[[2z1t]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2z1t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z1T FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z1t OCA], [https://pdbe.org/2z1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z1t RCSB], [https://www.ebi.ac.uk/pdbsum/2z1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z1t ProSAT]</span></td></tr>
-
{{STRUCTURE_2z1t| PDB=2z1t | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q72F88_DESVH Q72F88_DESVH]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z1/2z1t_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z1t ConSurf].
 +
<div style="clear:both"></div>
-
'''Crystal Structure of Hydrogenase Maturation Protein HypE'''
+
==See Also==
-
 
+
*[[HypA%2C HypB%2C HypC%2C HypD%2C HypE and HypF 3D structures|HypA%2C HypB%2C HypC%2C HypD%2C HypE and HypF 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The hydrogenase maturation protein HypE serves an essential function in the biosynthesis of the nitrile group, which is subsequently coordinated to Fe as CN(-) ligands in [Ni-Fe] hydrogenase. Here, we present the crystal structures of HypE from Desulfovibrio vulgaris Hildenborough in the presence and in the absence of ATP at a resolution of 2.0 A and 2.6 A, respectively. Comparison of the apo structure with the ATP-bound structure reveals that binding ATP causes an induced-fit movement of the N-terminal portion, but does not entail an overall structural change. The residue Cys341 at the C terminus, whose thiol group is supposed to be carbamoylated before the nitrile group synthesis, is completely buried within the protein and is located in the vicinity of the gamma-phosphate group of the bound ATP. This suggests that the catalytic reaction occurs in this configuration but that a conformational change is required for the carbamoylation of Cys341. A glutamate residue is found close to the thiol group as well, which is suggestive of deprotonation of the carbamoyl group at the beginning of the reactions.
+
[[Category: Desulfovibrio vulgaris str. Hildenborough]]
-
 
+
[[Category: Large Structures]]
-
==About this Structure==
+
[[Category: Higuchi Y]]
-
2Z1T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z1T OCA].
+
[[Category: Shomura Y]]
-
 
+
-
==Reference==
+
-
Crystal structures of hydrogenase maturation protein HypE in the Apo and ATP-bound forms., Shomura Y, Komori H, Miyabe N, Tomiyama M, Shibata N, Higuchi Y, J Mol Biol. 2007 Sep 28;372(4):1045-54. Epub 2007 Jul 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17706667 17706667]
+
-
[[Category: Desulfovibrio vulgaris]]
+
-
[[Category: Single protein]]
+
-
[[Category: Higuchi, Y.]]
+
-
[[Category: Shomura, Y.]]
+
-
[[Category: Alpha-beta fold]]
+
-
[[Category: Beta barrel]]
+
-
[[Category: Lyase]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 19:50:11 2008''
+

Current revision

Crystal Structure of Hydrogenase Maturation Protein HypE

PDB ID 2z1t

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2z1t"
Personal tools