2zew

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[[Image:2zew.jpg|left|200px]]
 
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==Family 16 Cabohydrate Binding Domain Module 1==
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The line below this paragraph, containing "STRUCTURE_2zew", creates the "Structure Box" on the page.
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<StructureSection load='2zew' size='340' side='right'caption='[[2zew]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zew]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobius_polysaccharolyticus Caldanaerobius polysaccharolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZEW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_2zew| PDB=2zew | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zew OCA], [https://pdbe.org/2zew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zew RCSB], [https://www.ebi.ac.uk/pdbsum/2zew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zew ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9ZA17_9THEO Q9ZA17_9THEO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ze/2zew_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zew ConSurf].
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<div style="clear:both"></div>
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'''Family 16 Cabohydrate Binding Domain Module 1'''
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==See Also==
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Enzymes that hydrolyze complex polysaccharides into simple sugars are modular in architecture and consist of single or multiple catalytic domains fused to targeting modules called carbohydrate binding modules (CBMs). CBMs bind to their ligands with high affinity, and increase the efficiency of the catalytic components by targeting the enzymes to its substrate. Here, we utilized a multi-disciplinary approach to characterize each of the two family 16 carbohydrate binding domain component of the highly active mannanase from the thermophile T. polysaccharolyticum. These represent the first crystal structures of family 16 CBMs. Calorimetric analysis show that while these CBMs demonstrate high specificity towards ss-1,4 linked sugars, they can engage both cello- and manno-polysaccharides. In order to elucidate the molecular basis for this specificity and selectivity, we have determined high resolution crystal structures of each of the two carbohydrate binding modules, as well as of binary complexes of CBM16-1 bound to either mannopentaose or cellopentaose. These results provide detailed molecular insights into ligand recognition and yield a framework for rational engineering experiments designed to expand the natural repertoire of these targeting modules.
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[[Category: Caldanaerobius polysaccharolyticus]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Bae B]]
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2ZEW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZEW OCA].
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[[Category: Nair SK]]
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==Reference==
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Molecular basis for the selectivity and specificity of ligand recognition by the family 16 carbohydrate binding modules from thermoanaerobacterium polysaccharolyticum manA., Bae B, Ohene-Adjei S, Kocherginskaya S, Mackie RI, Spies MA, Cann IK, Nair SK, J Biol Chem. 2007 Nov 29;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18025086 18025086]
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[[Category: Single protein]]
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[[Category: Thermomicrobium roseum]]
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[[Category: Bae, B.]]
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[[Category: Nair, S K.]]
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[[Category: Carbohydrate binding module]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:11:14 2008''
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Current revision

Family 16 Cabohydrate Binding Domain Module 1

PDB ID 2zew

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